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lecture_notes:05-27-2011

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lecture_notes:05-27-2011 [2011/05/27 21:10]
eyliaw created
lecture_notes:05-27-2011 [2011/05/28 03:25]
karplus mapping 454_run3 not needed.
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 ====== To Do ====== ====== To Do ======
-We went over some things that need updating in the wiki. +We went over some things that need updating in the wiki and plans for this weekend. 
-  * Document [[computer_resources:​bin|bin]] folder ​+ 
 +  * Document ​scripts added in [[computer_resources:​bin|bin]] folder
 + 
 + 
 +  * Find out which lanes in 454 run 3 are banana slug runs. But it turns out that this is not necessary: run "​3"​ is not a separate run, but just run2 plus the non-banana-slug reads in other lanes. 
 +     * Try mapping with Newbler or BWA. 
 +     * BLAST/BLAT it. 
 +  * Find insert lengths for the SeqPrep + Quake corrected Illumina data. 
 +  * SOAPdenovo assembly try 1: 
 +     * Only on Illumina data. 
 +  * try 2: 
 +     * Rank 1: All Illumina data for contig building. 
 +     * Rank 2: 454 data for scaffolding. 
 +  * try 3: 
 +     * Illumina + 454 data as rank 1. 
 +  * # If insert length is negative, don't treat them as PE reads. 
 +  * Newbler assemblies. 
 +  * Take another look at Barcode of Life mapping: [[http://​www.ncbi.nlm.nih.gov/​Taxonomy/​Utils/​wprintgc.cgi#​SG5|Invertebrate mitochondrial translation table]] 
 + 
 +  * Ed presenting paper on Wednesday. ​ Get paper over the weekend.
lecture_notes/05-27-2011.txt · Last modified: 2015/09/14 18:40 by 68.180.230.228