lecture_notes:05-13-2011
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lecture_notes:05-13-2011 [2011/05/13 23:39] eyliaw [Template Length] |
lecture_notes:05-13-2011 [2011/06/13 00:43] karplus [Insert Length Analysis] added pointer to main discussion |
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| - | ====== Insert Length Analysis ====== | + | ====== Insert Length Analysis (lecture notes) ====== |
| We discussed the results from mapping the Illumina run 2 reads to the 454 reads using [[bioinformatic_tools:bwa|BWA]]. | We discussed the results from mapping the Illumina run 2 reads to the 454 reads using [[bioinformatic_tools:bwa|BWA]]. | ||
| + | There is a more complete analysis at [[bioinformatic_tools:bwa#determining_paired-end_insert_size|Determining Paired-end Insert Size]]. | ||
| ===== Template Length ===== | ===== Template Length ===== | ||
| Line 12: | Line 12: | ||
| ===== 454 Coverage ===== | ===== 454 Coverage ===== | ||
| - | One possible explanation for misshaped distribution is that BWA had difficulty aligning the Illumina reads to the reference due to the possible overlap of the 454 reads. We calculated the expected number of overlaps in the 454 reads. | + | One possible explanation for misshapen distribution is that BWA had difficulty aligning the Illumina reads to the reference due to the possible overlap of the 454 reads. We calculated the expected number of overlaps in the 454 reads. |
| <code> | <code> | ||
| R = # of 454 reads ( ~500,000 ) | R = # of 454 reads ( ~500,000 ) | ||
lecture_notes/05-13-2011.txt · Last modified: 2015/09/12 02:47 by 5.9.83.211
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