lecture_notes:03-30-2011
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lecture_notes:03-30-2011 [2011/03/30 23:16] eyliaw |
lecture_notes:03-30-2011 [2011/03/30 23:20] eyliaw [Sanger capillary] |
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| Inputs: Sequencing data from various machines. Some of the characteristics of these machines/techniques: | Inputs: Sequencing data from various machines. Some of the characteristics of these machines/techniques: | ||
| ==== Sanger capillary ==== | ==== Sanger capillary ==== | ||
| - | ~800bp reads[(cite:sangerwiki>http://en.wikipedia.org/wiki/Microfluidic_Sanger_sequencing)]. | + | * ~800bp reads[(cite:wikisanger>http://en.wikipedia.org/wiki/Microfluidic_Sanger_sequencing)]. |
| - | Q (quality value) ~30 | + | * Q (quality value) ~30 |
| - | Expensive (~$1/read) because primers must be attached to each read. | + | * ~$1/read, expensive because primers must be attached to each read. |
| ==== 454 ==== | ==== 454 ==== | ||
| - | ~500bp reads[(cite:454wiki>http://en.wikipedia.org/wiki/454_Life_Sciences)]. | + | * ~400bp reads[(cite:wiki454>http://en.wikipedia.org/wiki/454_Life_Sciences)]. |
| - | Pyrosequencing | + | * Q ~20 |
| - | $5000/run/~1m reads | + | * Pyrosequencing |
| + | * $5000/run/~1m reads, no downscaling. | ||
| ==== SoLiD ==== | ==== SoLiD ==== | ||
lecture_notes/03-30-2011.txt · Last modified: 2011/04/01 19:20 by svohr
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