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====== Brief overview of goals and data input characteristics ====== Kevin laid out some of the logistics of the class. A broad goal: For each chromosome in the slug, we want the full sequence in DNA bases. Since it is unlikely to be completed in the timeframe of one quarter, some smaller goals: build contigs and have an idea of the scaffold to arrange the contigs in. Inputs: Sequencing data from various machines. Some of the characteristics of these machines/techniques: ==== Sanger capillary ==== ~800bp reads[(cite:sangerwiki>http://en.wikipedia.org/wiki/Microfluidic_Sanger_sequencing)]. Q (quality value) ~30 Expensive (~$1/read) because primers must be attached to each read. ==== 454 ==== ~500bp reads[(cite:454wiki>http://en.wikipedia.org/wiki/454_Life_Sciences)]. Pyrosequencing $5000/run/~1m reads ==== SoLiD ==== ==== Illumina ==== ==== Ion Torrent ==== ==== Pac Bio ==== ===== References ===== <refnotes>notes-separator: none</refnotes> ~~REFNOTES cite~~
