Kevin laid out some of the logistics of the class.

A broad goal: For each chromosome in the slug, we want the full sequence in DNA bases. Since it is unlikely to be completed in the timeframe of one quarter, some smaller goals: build contigs and have an idea of the scaffold to arrange the contigs in.

Inputs: Sequencing data from various machines. Some of the characteristics of these machines/techniques:

~800bp reads[1]. Q (quality value) ~30 Expensive (~$1/read) because primers must be attached to each read.

~500bp reads[1]. Pyrosequencing $5000/run/~1m reads