$sga --help
Program: sga
Version: 0.10.13
Contact: Jared Simpson [js18@sanger.ac.uk]
Usage: sga <command> [options]
Commands:
preprocess filter and quality-trim reads
index build the BWT and FM-index for a set of reads
merge merge multiple BWT/FM-index files into a single index
bwt2fa transform a bwt back into a set of sequences
correct correct sequencing errors in a set of reads
fm-merge merge unambiguously overlapped sequences using the FM-index
overlap compute overlaps between reads
assemble generate contigs from an assembly graph
oview view overlap alignments
subgraph extract a subgraph from a graph
filter remove reads from a data set
rmdup duplicate read removal
gen-ssa generate a sampled suffix array for the given set of reads
scaffold generate ordered sets of contigs using distance estimates
scaffold2fasta convert the output of the scaffold subprogram into a fasta file
gapfill fill intra-scaffold gaps
Variant Calling Commands:
graph-diff compare reads to find sequence variants
graph-concordance check called variants for representation in the assembly graph
rewrite-evidence-bam fill in sequence and quality information for a variant evidence BAM
haplotype-filter filter out low-quality haplotypes
somatic-variant-filters filter out low-quality variants
Experimental commands:
preqc perform pre-assembly quality checks on a set of reads
stats print summary statistics about a read set
filterBAM filter out contaminating mate-pair data in a BAM file
cluster find clusters of reads belonging to the same connected component in an assembly graph
kmer-count extract all kmers from a BWT file
Report bugs to js18@sanger.ac.uk