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archive:specific_task [2015/05/13 06:15]
nsaremi
archive:specific_task [2015/08/04 03:32] (current)
68.180.228.52 ↷ Links adapted because of a move operation
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 |Task| Assignee | Status | Notes| |Task| Assignee | Status | Notes|
-| Plot contig size vs total genome size for assemblies | | | | +|Install Maker| Houser | Complete | Maker is installed, see information [[archive:​maker|here]] | 
-| Ask UCSF about the discrepancy in the numbers of reads | | | I don't see any discrepancy in the number of reads, perhaps this can be removed? ​ |  +|Assemble repeat genome with RepArk|Team 1|In progress|Charles has started a test run using the original Hiseq and Miseq reads| 
-Analyze SeqPrep results ​| | | |  +|Run GapCloser on SOAP denovo assembly|Charles|In progress| Use SOAP denovo'​s external gap closer. Chose a reasonable name for new assembly. | 
-Plot contig size histogram for current assembly (SOAPdenovo2 results) | Markello | In progress | | +|Find virus contigs and use PRICE to expand to full viruses | Cole,​jolespin | | jolespin virus assembly progress can be accessed at [[archive:​jolespin_virus]]| 
-| Plot scaffold size histogram for current assembly (SOAPdenovo2 results) | Markello | In progress | | +| Mate-pair adapter and linker removal| ​ | | | 
-| Find cost of creating a tagmentase library| Dudek | | ~$50 | +| Measuring insert size for each mate-pair library | unassigned | | | 
-| Merge/trim new data (SeqPrep) ​| Hussain | in progress ​| | +| Making Seqprep, adapter removed, end-trimmed library files | unassigned | | | 
-Analyze new data (preqc) ​Richardson ​In progress ​| |   +| Blast long contigs and analyze results | unassigned| | Check the browser for the 30 longest contigs from each group with contigs|  
-| Error Correction new data| McGovern (Musket) & Richardson (BLESS/​racer) | In progress | These programs have not yet been run succesfully... ​|+| Find genes in the mitochondrial genome | unassigned | | The genome is on the browser, find it here https://​banana-slug.soe.ucsc.edu/​banana_slug_genome_browser |  
 +| Use PRICE to get mitochondrial genome as single contig | Natasha Dudek? | | | 
 +| Plot contig size vs total genome size for assemblies | unassigned ​| | | 
 +| Ask UCSF about the discrepancy in the numbers of reads | unassigned ​| | I don't see any discrepancy in the number of reads, perhaps this can be removed? ​ |  
 +Fastqc/​kmergenie on SeqPrepped data unassigned ​waiting on previous steps | |  
 +End trim SeqPrepped ​data | Hussain | | |  
 +Error correct SeqPrepped ​data | McGovern ​waiting on previous step | |  
 +| Error Correction new data| McGovern (Musket) | In progress |  |
 | Install bowtie | Chaves | In progress |/​campusdata/​gchaves/​Bowtie/​bowtie2-2.2.5 (Not yet running successfully)| | Install bowtie | Chaves | In progress |/​campusdata/​gchaves/​Bowtie/​bowtie2-2.2.5 (Not yet running successfully)|
 +| UCSC Genome browser hub | Eisenhart | Complete | https://​banana-slug.soe.ucsc.edu/​banana_slug_genome_browser | 
 +| Plot contig size histogram for current assembly (SOAPdenovo2 results) | Markello | Complete | |
 +| Plot scaffold size histogram for current assembly (SOAPdenovo2 results) | Markello | Complete | |
 +| Find cost of creating a tagmentase library| Dudek | | ~$50 |
 +| Merge/trim new data (SeqPrep) | Hussain | Completed | See wiki page for each data set for details |
 +| Analyze new data (preqc) | Richardson | Completed | See wiki page for each data set for details | 
 | Preqc on new mate pair SW041 SW042| Saremi | Completed | | | Preqc on new mate pair SW041 SW042| Saremi | Completed | |
 | Update the wiki to include mate pair meta data | Eisenhart | completed |  | Update the wiki to include mate pair meta data | Eisenhart | completed | 
-| Install CEGMA dependencies | Markello | In progress ​Relying on IT to fix issues with completing compilation ​of wise2 (genewise) package which CEGMA requires ​|+| Install CEGMA dependencies | Markello | Completed ​Issue discovered: glib required for complete installation of latest version ​of genewise ​(CEGMA dependency) on head node, will use current installation of genewise. ​|
 | Create a file containing contigs longer than 5kb | Eisenhart | completed | file is here /​campusdata/​BME235/​S15_assemblies/​DiscovarDeNovo/​UCSF50%run/​bigContigs.fa | | Create a file containing contigs longer than 5kb | Eisenhart | completed | file is here /​campusdata/​BME235/​S15_assemblies/​DiscovarDeNovo/​UCSF50%run/​bigContigs.fa |
 | Plot scaffold size histogram for current assembly (Discovar de novo results) | Dudek | Completed | 10% and the new UCSF 50% are up on the discovar team page | | Plot scaffold size histogram for current assembly (Discovar de novo results) | Dudek | Completed | 10% and the new UCSF 50% are up on the discovar team page |
-| Map lucigen mates against scaffolds ​to estimate insert size | Dudek | in progress ​ |+| Map lucigen mates against scaffolds | Dudek | completed ​See sam file at /​campusdata/​ndudek/​mapping_lucigen/​5kb_plus_discovar/​IJS8_mates_vs_discovar_bigContigs_5000.sam ​|
 | Analyze new data (fastqc) | Saremi |Completed | /​campusdata/​BME235/​S15_assemblies/​SOAPdenovo2/​Fastqc/​UCSF_BS-MK_fastqc /​campusdata/​BME235/​S15_assemblies/​SOAPdenovo2/​Fastqc/​UCSF_BS-tag_fastqc |  ​ | Analyze new data (fastqc) | Saremi |Completed | /​campusdata/​BME235/​S15_assemblies/​SOAPdenovo2/​Fastqc/​UCSF_BS-MK_fastqc /​campusdata/​BME235/​S15_assemblies/​SOAPdenovo2/​Fastqc/​UCSF_BS-tag_fastqc |  ​
 | Adapter trim UCSF SW018 and SW019 libraries (skewer), remove duplicates (fastUniq)| Chaves & Dudek | Completed |/​campusdata/​BME235/​S15_assemblies/​DiscovarDeNovo/​UCSF_reads (Read 1 Sequencing Primer: 5' ACACTCTTTCCCTACACGACGCTCTTCCGATCT 3'; Read 2 Sequencing Primer: 5' GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT 3)| | Adapter trim UCSF SW018 and SW019 libraries (skewer), remove duplicates (fastUniq)| Chaves & Dudek | Completed |/​campusdata/​BME235/​S15_assemblies/​DiscovarDeNovo/​UCSF_reads (Read 1 Sequencing Primer: 5' ACACTCTTTCCCTACACGACGCTCTTCCGATCT 3'; Read 2 Sequencing Primer: 5' GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT 3)|
-|SSpace on discovar de novo assembly fasta | Eisenhart | In process ​queued ​+|SSpace on discovar de novo assembly fasta | Eisenhart | Complete ​Results are posted here , https://​banana-slug.soe.ucsc.edu/​team_5_page:​sspacesummaryfile |  
 +| Map reads against 2012 mitochondrion assembly | Dudek | Completed for UCSF SW018 and SW019 reads | I did the mapping both for adapter-trimmed and adapter-trimmed & duplicate-free reads. The sam files are available at: /​campusdata/​BME235/​mitochondrion ​|
  
archive/specific_task.1431497722.txt.gz · Last modified: 2015/05/13 06:15 by nsaremi