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archive:lab_protocols:karyotyping [2011/03/29 00:29] eyliaw created |
archive:lab_protocols:karyotyping [2015/09/02 16:57] (current) ceisenhart ↷ Page moved from lab_protocols:karyotyping to archive:lab_protocols:karyotyping |
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====== Karyotyping ====== | ====== Karyotyping ====== | ||
Karyotyping should be done during the metaphase cycle, when the chromosomes are distinct and easily counted. To obtain a sample with a good number of cells in metaphase, typically blood or skin cells are collected. Some drugs are used to stimulate division and lock cells into metaphase: see [[http://arbl.cvmbs.colostate.edu/hbooks/genetics/medgen/chromo/cytotech.html|Preparing a Karyotype]]. | Karyotyping should be done during the metaphase cycle, when the chromosomes are distinct and easily counted. To obtain a sample with a good number of cells in metaphase, typically blood or skin cells are collected. Some drugs are used to stimulate division and lock cells into metaphase: see [[http://arbl.cvmbs.colostate.edu/hbooks/genetics/medgen/chromo/cytotech.html|Preparing a Karyotype]]. | ||
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+ | Another procedure done here: [[http://faculty.virginia.edu/xtropicalis/KaryotypeXtropicalis.htm|Karyotyping X. tropicalis]] to karyotype a species of frog, simply stains cells in the tadpoles, which are already actively dividing. |