Banana Slug Genomics

Prepared by Akram Tariq Sial

Date:

Overview: This sample was prepared according to manufacturer's recommendations. [Add link to illumina protocol]

Fermentas O'generuler 50bp ladder used in these gels. Lanes 1 & 6

1µg of 98.0 ng/µl genomic DNA was sheared using either Covaris or a Nebulizer. Lanes 3&4: Banana Slug DNA sheared using Covaris. Lanes 5&6: Banana Slug DNA sheared using a Nebulizer. Both were cut between 250-375 basepairs.

Bioanalyzer results of shearing distributions and final amplified libraries. Covaris and Nebulizer shearing are in lanes 1 and 2 respectively. Amplified and size selected libraries for Nebulizer and Covaris in lanes 3 and 4 respectively.

The results indicate the final nebulizer library ranges from 202-320bp in distribution. The final covaris library ranges from 225-263bp in length.

Each stage indicates a different distribution in the length of the paired-end reads. The original size selection cut indicates a fragment size of 250-375. However, the bioanalyzer results indicate the final size selected fragment is much shorter at 200-320 bp for a final distribution between 100-230 bp.

Each lane contained a different concentration of starting material used in bridge amplification.

These different starting quantities of DNA are reflected in the number of clusters generated and the total reads outputted for each lane. Additionally, a different distribution of distance for the paired-end reads are different for lanes 1-3 and 5-8

*Data from lane 4 of the illumina run SHOULD NOT be used for assembly