For the Spring 2015 class the banana slug genome project was funded by a successful crowd-funding campaign (https://crowdfund.ucsc.edu/project/5424db950920652635292bae). Below is the complete budget for the project, the items are arranged in no particular order. Please note that we owe some things to the donors so after these fees the total budget available to us is $20,156 out of the full $21,443.
|Donor gift fees||The amount that UCSC charged for collecting the donations (6%)||$1,287.00|
|DNA extraction OMEGA mollusc DNA kit||Technician time for generating 3 DNA extracts||$109.00|
|RNA extraction OMEGA molluscRNA kit||Technician time for generating 30 RNA extracts||$445.00|
|Shotgun library Reagents||Technician time for generating 2 shotgun Illumina libraries||$397.00|
|Mate-pair library Lucigen mate-pair library kit||User-provided reagents; technician time for generating||$3,647.66|
|MiSeq sequencing||Small-scale miseq sequencing for library QC||$57.00|
|MiSeq sequencing||2×300 full MiSeq run||$2,117.00|
|HiSeq sequencing||2×100 library generated on a single HiSeq lane with 597bp insert size||$2000.00|
|HiSeq sequencing||2×100 library generated on a single HiSeq lane with 374bp insert size||$2000.00|
|HiSeq sequences||2×250 library pools at UCSF; 2 lanes||$5600|
Contact Kevin Karplus with questions.