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====== Banana slug genomics ====== | ====== Banana slug genomics ====== | ||
- | ===== Reborn - Spring 2015 ===== | + | {{http://users.soe.ucsc.edu/~karplus/cyberslugs/slug-dreaming-300x400.jpg }}((The Cyberslug is **not** licensed under Creative Commons. It is licensed exclusively to the Jack Baskin School of Engineering at the University of California, Santa Cruz, and may not be used by others. Furthermore, the slug-dreaming icon here is only for use by the Banana Slug Genomics project—contact [[https://www.soe.ucsc.edu/people/karplus | Kevin Karplus]] for permission to use it.)) |
- | Welcome back, Banana Slug Genomics! Following the successful [[https://crowdfund.ucsc.edu/project/5424db950920652635292bae|crowdfunding campaign]], this class and effort will be rebooted. | ||
- | * [[Spring 2015 syllabus]] The lecture schedule and registered note takers. | + | **This is the home page for the Banana Slug Genomics wiki. ** |
- | * [[Data overview]] Raw data and associated analysis. | + | |
- | * [[Teams]] Each team is attempting an assembly with a different program. | + | |
- | * [[Style Guide|Complete guide for modifying the wiki]] | + | |
- | * [[computer_resources:campusrocks|Complete guide to campusRocks and SGE]] | + | |
- | * [[Budget]] | + | |
- | * [[Specific task]] | + | |
- | * [[Banana slug genome browser]] A guide for accessing and setting up the banana slug hub. | + | |
- | ===== Important pages of this wiki ===== | + | **We are working to assemble and annotate the genome of the UCSC mascot //Ariolimax dolichophallus//, the slender banana slug. Additionally we are collecting and hosting as much information as we can about the biology of the banana slug.** |
- | * [[Computer resources]] describes the computers and file systems available for this class, how to get an account, how the directories are organized, how to get file permissions set correctly, and so forth. | + | |
- | * [[Style guide]] will have suggestions for organizing content on the wiki. | + | |
- | * [[Lecture notes]] will have pointers to notes taken by the students for each lecture. Two students will be designated each lecture to take notes, but others are encouraged to add their own notes to the wiki pages. | + | |
- | * [[Other mollusk genomes]] has a list of other mollusks that have been sequenced, and where the data for them is. Prior mollusk genomes may be very useful in annotating the banana slug. | + | |
- | * [[RNA]] | + | |
+ | Researchers and staff at UCSC created this [[https://www.youtube.com/watch?t=11&v=hF8IkJpq6OI | video ]] for the 2014 crowdfunding campaign. See how the project started and follow along at a high level, a great place to start for those uninitiated in genomics! | ||
- | ====Team pages and results ==== | + | This project is hosted at UCSC as a graduate class BME235. All information is publicly accessible and free to use, please cite the authors when possible and the wiki as a whole. Please contact [[https://www.soe.ucsc.edu/people/karplus | Kevin Karplus]] with general questions. For questions on specific assemblies please identify the team that created the assembly and email them directly. |
- | Each team is attempting to assemble the banana slug genome with a different assembly program. The results are posted in the team pages below. | + | ===== About the slug ===== |
- | | Team page | N50 | | + | The Ariolimax Dolicophallus is a relatively unstudied species. Here we aim to provide concise and accurate biological information about the Ariolimax Dolicophallus. |
- | |[[team_1_page| Meraculous: Team 1 page]]| | | + | |
- | |[[soapdenovo2|SOAPdenovo2: Team 2 page]]| **5,554** | | + | |
- | |[[0.10.13|SGA: Team 3 page]]| | | + | |
- | |[[team_4_page| ABySS: Team 4 page]]| | | + | |
- | |[[team_5_page| Discovar de novo: Team 5 page]]| 3,979 | | + | |
+ | [[About the slug | Biological information about the slug ]] | ||
+ | ===== Data ===== | ||
+ | Links to the raw data, as well as processed data is provided [[Data overview | here]] | ||
+ | [[Data overview | Access to the raw and processed data]] | ||
+ | ===== Assemblies ===== | ||
+ | The assemblies are available for annotation, merging and other bioinformatics uses. | ||
+ | [[Full_genome_assemblies | Full genome assemblies ]] | ||
- | ---- | + | [[Mitochondrion_assemblies | Mitochondrion assemblies ]] |
+ | ===== Post-assembly analysis ===== | ||
+ | Our analysis of the completed assemblies. Includes the [[Banana slug genome browser |UCSC track hub for the banana slug]], analysis of heterozygosity, annotation results, and [Euchromatin and Heterochromatin | analysis of banana slug euchromatin and heterochromatin]]. | ||
- | {{http://users.soe.ucsc.edu/~karplus/cyberslugs/slug-dreaming-300x400.jpg }}((The Cyberslug is **not** licensed under Creative Commons. It is licensed exclusively to the Jack Baskin School of Engineering at the University of California, Santa Cruz, and may not be used by others. Furthermore, the slug-dreaming icon here is only for use by the Banana Slug Genomics project—contact Kevin Karplus for permission to use it.)) | + | [[Banana slug genome browser] | UCSC Genome Browser Banana slug track hub] |
+ | [[Euchromatin and Heterochromatin | Banana slug euchromatin and heterochromatin]] | ||
- | This is the home page for the Banana Slug Genomics wiki. | ||
- | Material on these pages will mainly be created by students of the [[http://users.soe.ucsc.edu/~karplus/bme235|BME235 course]]. We hope to be able to assemble and annotate the genome of the UCSC mascot //Ariolimax dolichophallus//, the slender banana slug, as well as collecting all the information we can find about the biology of the banana slug. | ||
- | This wiki should be publicly readable, but editable only by people with UCSC School of Engineering computer accounts. | ||
- | Contact Kevin Karplus (karplus@soe.ucsc.edu) if you need to edit these pages and do not have such an account. | ||
- | In addition to page-specific discussions on each page, there is a separate [[http://forums.soe.ucsc.edu/viewforum.php?f=102|forum]] for discussions about the class. The discussion forum is intended for more transient discussions than this wiki---such as where new material should go into the wiki, discussions of re-organizing the wiki or alerts about time-sensitive material. | ||
- | ===== Old pages of this wiki ===== | + | |
- | These links are several years old. Due to the speed in which bioinformatics moves, most if not all of this information will be outdated. | + | |
- | * [[computer_resources:data|Data available]] lists the datasets that we have to assemble and annotate the genome. The data will not appear on the wiki itself (due to the size of the files), but descriptions of it and pointers to the files will. | + | ===== About the class ===== |
- | * [[computer_resources:assemblies|Assemblies]] lists the attempted assemblies (separated into subdirectories by organism). | + | The project is supported at UCSC as the BME235 Banana Slug Genomics class. More information about the class can be found in the [[About the class | general information]] link. To see which students have worked on the project view the [[Students | students ]] link. The budget for the project is publicly viewable using the [[Budget | budget]] link. Old pages, pages that do not follow the style guide, and pages that cannot be assimilated into the main wiki are archived [[Archive | here]]. |
- | * [[Lab protocols]] will describe how the slugs were collected, the DNA extracted, and all the wet-lab work was done to produce the data files. | + | |
- | * [[Sequencing technologies]] lists the platforms used for getting data on the banana slug. From there, we'll have pages on each technology explaining how it works, what sort of data it produces, what its common errors modes are, and other information needed to work with the data. | + | [[About the class | General information ]] |
- | * [[Bioinformatic tools]] lists the available tools for assembly and annotation. Each tool will have its own web page describing its strengths and weaknesses, and what we managed to do using that tool. | + | |
- | * [[Banana slug biology]] collects the information we can find about the biology of //Ariolimax dolichophallus// (and other //Ariolimax// species). | + | [[Students | Students ]] |
- | * [[Banana slugs in popular culture]] collects random information about banana slugs that can't really be classified as "biology". This will probably contain mainly information about the slug as the UCSC mascot. | + | |
- | * [[Contributors]] will list the main contributors to this project (providing pictures and links to their own pages as appropriate). | + | [[Budget | Budget]] |
- | * [[Review Articles]] will list helpful review articles discovered. | + | |
+ | [[Archive | Archive]] | ||
+ | |||
+ | Currently we **are not** accepting donations. Thanks to all our current and previous donors! | ||
+ | |||
+ | ---- | ||
+ | |||
+ | This wiki is publicly readable, but editable only by people with UCSC School of Engineering computer accounts. | ||
+ | Contact [[https://www.soe.ucsc.edu/people/karplus | Kevin Karplus]] if you need to edit these pages and do not have such an account. | ||
+ | |||
+ | In addition to page-specific discussions on each page, there is a separate [[http://forums.soe.ucsc.edu/viewforum.php?f=102|forum]] for discussions about the class. The discussion forum is intended for more transient discussions than this wiki---such as where new material should go into the wiki, discussions of re-organizing the wiki or alerts about time-sensitive material. | ||