lecture_notes:mar31
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lecture_notes:mar31 [2010/04/01 10:25] svasili |
lecture_notes:mar31 [2010/04/01 10:37] svasili |
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| __Sequencing by synthesis/Pyrosequencing__\\ | __Sequencing by synthesis/Pyrosequencing__\\ | ||
| - | Involves synthesizing complementary strand of DNA to the template DNA strand to be sequenced. Four enzymes are used in this method - DNA polymerase, ATP sulfyrylase, Luciferase, and Apyrase. The light generated is captured as a signal which detects the base incorporated. Currently the read length is approximately 400 nucleotides. Incorporation of homopolymer A is problematic.\\ | + | Involves synthesizing complementary strand of DNA to the template DNA strand to be sequenced. Four enzymes are used in this method - DNA polymerase, ATP sulfyrylase, Luciferase, and Apyrase. The light generated is captured as a signal which detects the base incorporated. Currently the read length is approximately 400 nucleotides. Incorporation of homopolymer A is found to be problematic.\\ |
| Further description of how the 4 enzymes work in this process can be found here[[http://en.wikipedia.org/wiki/Pyrosequencing]].\\ | Further description of how the 4 enzymes work in this process can be found here[[http://en.wikipedia.org/wiki/Pyrosequencing]].\\ | ||
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| - | Also we meet in the science library this Friday. | + | Also we meet in the science library (instructions room near periodicals) this Friday. |
lecture_notes/mar31.txt ยท Last modified: 2010/04/01 10:44 by svasili
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