User Tools

Site Tools


lecture_notes:mar31

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revision Previous revision
lecture_notes:mar31 [2010/04/01 10:37]
svasili
lecture_notes:mar31 [2010/04/01 10:44] (current)
svasili
Line 35: Line 35:
 ABI SOLiD sequencing technology follows sequencing by ligation. In first ligation cycle, possible 1024 oligonucleotide probes compete for incorporation,​ followed by dephosphorylation,​ visualization,​ and cleavage. Same procedure is followed for the next ligation cycles. Every 5th round of cycle, the process is reset. In the next cycle, primer length is reduced by one base (n-1) to get overlap, and again in the subsequent cycles the primer length is n-2, n-3 and so on to determine the DNA sequence due to overlap. For further reading follow [[http://​sequencing.soe.ucsc.edu/​node/​20]].\\ ABI SOLiD sequencing technology follows sequencing by ligation. In first ligation cycle, possible 1024 oligonucleotide probes compete for incorporation,​ followed by dephosphorylation,​ visualization,​ and cleavage. Same procedure is followed for the next ligation cycles. Every 5th round of cycle, the process is reset. In the next cycle, primer length is reduced by one base (n-1) to get overlap, and again in the subsequent cycles the primer length is n-2, n-3 and so on to determine the DNA sequence due to overlap. For further reading follow [[http://​sequencing.soe.ucsc.edu/​node/​20]].\\
  
-**Mate-Pair** - The chances of damage to DNA increases with time. Hence, there is a high possibility of not getting the accurate sequence data from the other end of DNA. To avoid this, the Mate-Pair process involves the determination of sequence of DNA from the end towards the beginning and then from the beginning towards the end.\\+**Mate-Pairing** - The chances of damage to DNA increases with time. Hence, there is a high possibility of not getting the accurate sequence data from the other end of DNA. To avoid this, the Mate-Pairing ​process involves the determination of sequence of DNA from the end towards the beginning and then from the beginning towards the end.\\
   ​   ​
  
lecture_notes/mar31.txt ยท Last modified: 2010/04/01 10:44 by svasili