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lecture_notes:mar31 [2010/04/01 10:10] svasili |
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7-10 days for SOLiD.\\ | 7-10 days for SOLiD.\\ | ||
- | These platforms are classified into two :\\ | + | The underlying technolgies for the platforms :\\ |
* Sequencing by synthesis (SBS) : 454, Illumina/Solexa, Helicos, Pacbio, Charge based detection system Now Sequencing.\\ | * Sequencing by synthesis (SBS) : 454, Illumina/Solexa, Helicos, Pacbio, Charge based detection system Now Sequencing.\\ | ||
* Sequencing by ligation : SOLiD.\\ | * Sequencing by ligation : SOLiD.\\ | ||
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* Sequence reads over multiple chemistry cycles.\\ | * Sequence reads over multiple chemistry cycles.\\ | ||
* Read length not fixed.\\ | * Read length not fixed.\\ | ||
- | * The chemistry of Illumina sequencing plaform can be found at [[http://www.illumina.com/technology/sequencing_technology.ilmn]].\\ | + | * The chemistry of Illumina sequencing platform can be found at [[http://www.illumina.com/technology/sequencing_technology.ilmn]].\\ |
* Now Sequencing Platform (CMOS based sequencing platform) - Sequencing based on detection of charge when a base is incorporated. Link to the article published by Nader in this respect is [[http://www.pnas.org/content/103/17/6466]]. On an average this sequencing technology incorporates upto 2.5 nucleotides in 2 minutes and in 60 minutes upto 100 bases.\\ | * Now Sequencing Platform (CMOS based sequencing platform) - Sequencing based on detection of charge when a base is incorporated. Link to the article published by Nader in this respect is [[http://www.pnas.org/content/103/17/6466]]. On an average this sequencing technology incorporates upto 2.5 nucleotides in 2 minutes and in 60 minutes upto 100 bases.\\ |