lecture_notes:mar31
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lecture_notes:mar31 [2010/04/01 09:18] svasili |
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| These platforms are classified into two :\\ | These platforms are classified into two :\\ | ||
| * Sequencing by synthesis (SBS) : 454, Illumina/Solexa, Helicos, Pacbio, Charge based detection system Now Sequencing.\\ | * Sequencing by synthesis (SBS) : 454, Illumina/Solexa, Helicos, Pacbio, Charge based detection system Now Sequencing.\\ | ||
| - | * Sequencing by hybridization : SOLiD.\\ | + | * Sequencing by ligation : SOLiD.\\ |
| __Sequencing by synthesis/Pyrosequencing__\\ | __Sequencing by synthesis/Pyrosequencing__\\ | ||
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| * Read length not fixed.\\ | * Read length not fixed.\\ | ||
| * The chemistry of Illumina sequencing plaform can be found at [[http://www.illumina.com/technology/sequencing_technology.ilmn]].\\ | * The chemistry of Illumina sequencing plaform can be found at [[http://www.illumina.com/technology/sequencing_technology.ilmn]].\\ | ||
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| - | * Now Sequencing Platform (CMOS based sequencing platform) - Sequencing based on charge detection when a base is incorporated. On average incorporates upto 2.5 nucleotides in 2 minutes and in 60 minutes upto 100 bases. | + | * Now Sequencing Platform (CMOS based sequencing platform) - Sequencing based on detection of charge when a base is incorporated. Link to the article published by Nader in this respect is [[http://www.pnas.org/content/103/17/6466]]. On an average this sequencing technology incorporates upto 2.5 nucleotides in 2 minutes and in 60 minutes upto 100 bases.\\ |
| + | __Sequencing by Ligation__\\ | ||
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lecture_notes/mar31.txt · Last modified: 2010/04/01 10:44 by svasili
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