lecture_notes:mar31
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| * 454 - Small ~20micron beads capturing DNA loaded onto PicoTiter plate along with the required enzymes for sequencing. The chemistry of the 454 sequencing can be found in detail at [[http://454.com/products-solutions/how-it-works/sequencing-chemistry.asp]].\\ | * 454 - Small ~20micron beads capturing DNA loaded onto PicoTiter plate along with the required enzymes for sequencing. The chemistry of the 454 sequencing can be found in detail at [[http://454.com/products-solutions/how-it-works/sequencing-chemistry.asp]].\\ | ||
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| * Illumina/Solexa \\ | * Illumina/Solexa \\ | ||
| * Preparing DNA sample : Randomly fragment genomic DNA and ligate adapters to both ends of the fragments.\\ | * Preparing DNA sample : Randomly fragment genomic DNA and ligate adapters to both ends of the fragments.\\ | ||
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| * Sequence Colonies : In first chemistry cycle determine first base. Get rid of dye, otherwise chances of mis-incorporation.\\ | * Sequence Colonies : In first chemistry cycle determine first base. Get rid of dye, otherwise chances of mis-incorporation.\\ | ||
| * Sequence reads over multiple chemistry cycles.\\ | * Sequence reads over multiple chemistry cycles.\\ | ||
| - | * Read length not fixed. | + | * Read length not fixed.\\ |
| + | * The chemistry of Illumina sequencing plaform can be found at [[http://www.illumina.com/technology/sequencing_technology.ilmn]].\\ | ||
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| + | * Now Sequencing Platform (CMOS based sequencing platform) - Sequencing based on detection of charge when a base is incorporated. Link to the article published by Nader in this respect is [[http://www.pnas.org/content/103/17/6466]]. On an average this sequencing technology incorporates upto 2.5 nucleotides in 2 minutes and in 60 minutes upto 100 bases.\\ | ||
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lecture_notes/mar31.txt · Last modified: 2010/04/01 10:44 by svasili
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