lecture_notes:mar31
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| * 454 - Small ~20micron beads capturing DNA loaded onto PicoTiter plate along with the required enzymes for sequencing. The chemistry of the 454 sequencing can be found in detail at [[http://454.com/products-solutions/how-it-works/sequencing-chemistry.asp]].\\ | * 454 - Small ~20micron beads capturing DNA loaded onto PicoTiter plate along with the required enzymes for sequencing. The chemistry of the 454 sequencing can be found in detail at [[http://454.com/products-solutions/how-it-works/sequencing-chemistry.asp]].\\ | ||
| * Illumina/Solexa \\ | * Illumina/Solexa \\ | ||
| - | * Randomly fragment genomic DNA and ligate adapters to both ends of the fragments | + | * Preparing DNA sample : Randomly fragment genomic DNA and ligate adapters to both ends of the fragments.\\ |
| + | * Immobilize DNA to surface : Bind single stranded fragments randomly to the inside surface of the flow cell channels.\\ | ||
| + | * Bridge amplification.\\ | ||
| + | * Sequence Colonies : In first chemistry cycle determine first base. Get rid of dye, otherwise chances of mis-incorporation.\\ | ||
| + | * Sequence reads over multiple chemistry cycles.\\ | ||
| + | * Read length not fixed. | ||
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lecture_notes/mar31.txt · Last modified: 2010/04/01 10:44 by svasili
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