lecture_notes:mar31
Differences
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lecture_notes:mar31 [2010/04/01 03:41] jstjohn |
lecture_notes:mar31 [2010/04/01 08:04] svasili |
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| 2) Sequencing by hybridization : SOLiD.\\ | 2) Sequencing by hybridization : SOLiD.\\ | ||
| - | __Sequencing by synthesis__\\ | + | __Sequencing by synthesis/Pyrosequencing__\\ |
| - | Involves synthesizing complementary strand of DNA to the template DNA strand to be sequenced. Four enzymes are used in this method - DNA polymerase, ATP sulfyrylase, Luciferase, and Apyrase. The light generated is captured as a signal which detects the base incorporated. The read length is approximately 400 nucleotides.\\ | + | Involves synthesizing complementary strand of DNA to the template DNA strand to be sequenced. Four enzymes are used in this method - DNA polymerase, ATP sulfyrylase, Luciferase, and Apyrase. The light generated is captured as a signal which detects the base incorporated. Currently the read length is approximately 400 nucleotides.\\ |
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| bin/ | bin/ | ||
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| bin/scripts/ | bin/scripts/ | ||
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| bin/x86_64 | bin/x86_64 | ||
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| bin/[picky_program_directories] (store programs that require the proximity of helper scripts) | bin/[picky_program_directories] (store programs that require the proximity of helper scripts) | ||
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| data/ | data/ | ||
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| programs/ (store program source and test programs before adding them to bin) | programs/ (store program source and test programs before adding them to bin) | ||
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| lib/ | lib/ | ||
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| experiments/ (store trial runs while testing out settings etc) | experiments/ (store trial runs while testing out settings etc) | ||
lecture_notes/mar31.txt · Last modified: 2010/04/01 10:44 by svasili
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