lecture_notes:05-15-2015
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lecture_notes:05-15-2015 [2015/05/18 14:46] gepoliano created |
lecture_notes:05-15-2015 [2015/05/18 15:01] gepoliano |
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| - | SGA update | + | =====SGA update===== |
| + | -SGA is a memory efficient assembler | ||
| + | -It was possible to compute more compressed data | ||
| + | -The pipeline changed, since it was not easy to figure out how to run it | ||
| + | -It was necessary to make sure the parameters are running | ||
| + | -The group assembled one dataset, merged together | ||
| + | -SGA indexed each dataset separated | ||
| + | -Merging is complicated in a pairwise fashion, then two pairs were merged at a time | ||
| + | -Indexing all three sistinct submissions | ||
| + | -Pre-processed adapter trimming | ||
| + | -Duplicate-removal is later than indexing | ||
| + | -One issue the group found: SW018 and 19, same library are optical PCR duplicates that should be removed | ||
lecture_notes/05-15-2015.txt · Last modified: 2015/05/18 15:11 by gepoliano
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