lecture_notes:05-06-2015
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lecture_notes:05-06-2015 [2015/05/08 09:16] gepoliano created |
lecture_notes:05-06-2015 [2015/05/08 18:57] ndudek |
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| * Expression of specific alleles | * Expression of specific alleles | ||
| * Information about the process of RNA editing | * Information about the process of RNA editing | ||
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| + | The biggest difference between RNAseq and DNA sequencing is that different cells in the body will have different RNA expression levels for each gene, whereas every cell in the body will have the same DNA. | ||
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| * TopHat is a mapper for splice junctions | * TopHat is a mapper for splice junctions | ||
| * The junctions are the canonical splice sites, (GU/AG are favored) | * The junctions are the canonical splice sites, (GU/AG are favored) | ||
| - | * Reads are split into thirds | + | * Reads are split into thirds (note that your reads must be long enough that, when split into thirds, they still contain meaningful information) |
| * Aligns RNAseq reads using Bowtie | * Aligns RNAseq reads using Bowtie | ||
lecture_notes/05-06-2015.txt ยท Last modified: 2015/05/08 18:57 by ndudek
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