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lecture_notes:04-30-2010 [2010/05/02 06:04] galt created |
lecture_notes:04-30-2010 [2010/05/02 22:01] jstjohn |
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Should add a mappers section on the tools page. | Should add a mappers section on the tools page. | ||
- | Next week will be student presentations. | + | Next week will be student presentations. We need volunteers to do presentations. Especially the people who haven't yet presented on an assembler or mapper. |
Presentations should include the algorithms, | Presentations should include the algorithms, | ||
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Contiglengths.rdb can give an estimate of coverage. | Contiglengths.rdb can give an estimate of coverage. | ||
- | back to .join. | + | Back to .join.\\ |
- | 3 2 is the same as -2 -3 | + | 3 2 is the same as -2 -3\\ |
- | -7 20 === -20 7 | + | -7 20 === -20 7\\ |
- | If you flip the pair, the direction an order switch. | + | If you flip the pair, the direction an order switch.\\ |
- | If he has - - , he just flips it to have + +. | + | If he has - - , he just flips it to have + +.\\ |
- | chose to put the lower-number contig first. | + | Chose to put the lower-number contig first.\\ |
The last column is useful but misleading. | The last column is useful but misleading. | ||
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where would | where would | ||
- | ----------- | + | "-----------" |
- | ----------- | + | "-----------" |
- | + | "-------| |------" | |
- | -------| |------ | + | "----------" |
- | ---------- | + | "---------" |
- | --------- | + | |
If you pretend they were all at the average length, | If you pretend they were all at the average length, | ||
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Significance values are tough because of various problems. | Significance values are tough because of various problems. | ||
- | In one case, | + | In one case, ended up with 40 contigs and only 3 could be properly mapped. |
- | Ended up with 40 contigs and only 3 could be properly mapped. | + | |
For inversions, flip and look for reads across boundaries. | For inversions, flip and look for reads across boundaries. | ||
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===== Homework for Wednesday ===== | ===== Homework for Wednesday ===== | ||
- | Do the contig alignments from the .join file. | + | Do the contig alignments from the .join file.\\ |
(Kevin had already re-done the map-colorspace5.) | (Kevin had already re-done the map-colorspace5.) | ||