lecture_notes:04-28-2010
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lecture_notes:04-28-2010 [2010/05/02 05:34] galt |
lecture_notes:04-28-2010 [2010/05/02 05:42] galt |
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| use makefiles, not shell scripts! | use makefiles, not shell scripts! | ||
| - | SOLiD data formats:\\ | + | **Sanger quality info**\\ |
| + | Kevin found the location of the Sanger qual info.\\ | ||
| + | .as or something like that.\\ | ||
| + | 3 different files from 3 different runs.\\ | ||
| + | |||
| + | **SOLiD data formats**:\\ | ||
| .csfasta = colorspace with numbers\\ | .csfasta = colorspace with numbers\\ | ||
| .de = changes #s to letters (0123 -> ACGT) but it’s colors not numbers! very confusing.\\ | .de = changes #s to letters (0123 -> ACGT) but it’s colors not numbers! very confusing.\\ | ||
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| === MIRA === | === MIRA === | ||
| + | |||
| + | Mostly used the default settings. | ||
| + | |||
| + | mira-assembly1/ | ||
| + | |||
| + | Running is easy. | ||
| + | Parameters: fasta denovo, tell it which instruments it has (e.g. 454 etc). | ||
| Needs datafile named pog_in.[format].fa \\ | Needs datafile named pog_in.[format].fa \\ | ||
| - | sff_extract script to create .qual files | + | uses sff_extract script to create .fasta and .fasta.qual files \\ |
| + | and also the traceinfo_in.454.xml file. | ||
| - | created 30 contigs >=500 (largest contig 640k) \\ | + | Time: 1 hour plus. |
| - | but... upon mapping to the reference genome, \\ | + | |
| + | Created 621 contigs, 30 larger than 500. (largest contig 640k) \\ | ||
| + | The 500 cutoff it probably too large.\\ | ||
| + | 100 might me more reasonable.\\ | ||
| + | Total concensus size is good.\\ | ||
| + | But... upon mapping to the reference genome, \\ | ||
| it turns out that while it is making big contigs, it's producing a chimeric assembly, in which the contigs join genomic regions that are not truly adjacent. | it turns out that while it is making big contigs, it's producing a chimeric assembly, in which the contigs join genomic regions that are not truly adjacent. | ||
| - | it’s getting bigger contigs because it’s joining them incorrectly! \\ | + | It’s getting bigger contigs because it’s joining them incorrectly! \\ |
| - | this is very bad; worse even than a lot of small contigs \\ | + | This is very bad; worse even than a lot of small contigs \\ |
| + | |||
| + | Not DBG. Should find out more about how it actually works.\\ | ||
| + | Good to know how it works so you know what to do with the parameters. | ||
| + | |||
| + | Newbler may be able to take fasta+qual file. | ||
| + | |||
| + | Mira might be worth fussing with on the parameters a bit more if it looks like | ||
| + | it is doing a good job. | ||
| + | |||
| + | Mira probably can't handle large genomes due to memory. | ||
| + | Mira has a tool to estimate memory required. | ||
| + | For a 3.2G genome it will need 1.1TB ram. | ||
lecture_notes/04-28-2010.txt · Last modified: 2010/05/02 16:22 by karplus
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