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Guest lecture by John Pearse and Janet Leonard, from Long Marine Laboratory, University of California-Santa Cruz. They did research on Banana Slugs for several years and talked about their findings in the class.
John talked about habitat, color, general characteristics, predators, and taxonomy of hermaphrodites. Janet talked mostly about sexual behavior of slugs.
Jeffrey Long has slides from Janet Leonard and he will be uploading them.
Additional notes from Jonathan follow.
John originally worked with sea urchins and sea stars primarily.
Janet joined the lab about twelve years ago to study marine slugs.
She was interested in hermaphrodite mating behavior. Banana slugs' rare behavior of apophallation became a research focus. No one had
really studied banana slugs [academically] since the forties. Alice Bryant Harper (Aptos naturalist, works with Santa Cruz Museum of
Natural History) wrote The Banana Slug (1988), the best book on them.
Despite Chancellor Sinsheimer's desire to keep the sea lion as the UCSC mascot, students voted 95% for the banana slug.
There are just two complete mollusc genomes, [California sea hare and giant owl limpet], and neither are very complete.
Banana slugs habitat is very diverse. Though often found in conifer
forests and considered an animal of the Northwest (first found in
Washington or Oregon), they've been found in drier habitats: San Diego, Napa's McLaughlin Reserve (by small springs), abandoned rice
patties in the Sacramento River Delta, oceanside iceplants in Pacific Grove. High variation in the numbers you'll see on any day/site: none
They eat feces, hemlock, poison oak, mushrooms (reported but
John has not seen), sorrel, ferns, ice plants, humus soil. In the lab
they eat hamburger, cat food, apples, beans, zucchini, mushrooms, yams, lettuce and milk.
Colors may camouflage them, e.g. dead leaves often turn bright
yellow, the color of species in Santa Cruz and the SF Bay area. In
other areas you'll find spotted slugs – but they may be a different species.
There predators may include [seemed uncertain] garter snakes,
salamanders and newts, birds and some small mammals. It is possible
that some specific carnivorous snails and slugs eat banana slugs.
Aphallarion buttoni originally thought to be a different species
because no penes were found when dissected (late 19th century).
However, a Stanford professor later found some with penes and so sent students into the field to study. They observed apophallation. That
was the end of buttoni as a separate taxon. It became Ariolimax columbianus.
All banana slugs have an opening on the right side of the “head” for
defecation, breathing, and copulation. The only way to distinguish
species is by dissection of the genitalia. [See slide *Ariolimax Arilimax columbians genitalia* for overview of genitalia.] The
gonad has a mix of testes and ovaries, and they can play both roles at same time curing copulation. How is sperm kept separate during
copulation? It is not necessarily. They can fertilize themselves.
And aphalon are born without a penis [sometimes?].
Ariolimax Meadarion californicus is found in San Mateo county.
Santa Cruz has dolichophallus. [See slide comparing their
genitalia.] Mead thoought dolichophallus and californicus were sufficiently different to be a separate species.
A collaborator in Belgium has been sequencing banana slug
mitochrondrial DNA. They see at least five clades but cannot yet
connect them. ~“Morphologically distinct and molecularly distinct are not the same thing.” [See slide.]
Interestingly the distribution of the salamander genus Ensatia is
similar to that of banana slug [dolichophallus? – see slide]. Is
this a remnant of five million years ago when there were islands in the Monterey Bay? Morphologically distinct but molecularly
[mito. DNA] indistict suggests recent change.
Janet's interest is in sex selection. Eberhard's hypothesis: [That we
can ] classif[y] based on genitalia (as done with insects, spiders,
etc.) suggests the importance of sex selection. Genitalia differences in nearby counties are not explicable by natural
selection, e.g. how would NS explain [improved fitness by a different vaginal muscle in the same geographical area.]
The talk focused on courtship behavior: much effort/expense in banana slug mating.
Ariolimax stramineus courtship: antiparallel alignment of slugs is
standard. They line up right sides of their heads until the alignment
allows copulation. The first copulation takes place in about twenty minutes with subsequent occuring over about two hours. No apophallation in the
one shown on film.
Mating film for brachyphallus: This is one of the three under
Ariolimax Meadarian. Note the initial biting and head swinging
which seem to cause no damage. The biting helps line up the head regions. Banana slugs cannot reverse so the cirlcing helps get them
get into position for mating.
Mating film for californicus: Unilateral copulation (after two hours
biting, head swinging). It is hard to tell which plays the role of
male and female but Janet thinks they alternate over a copulation session.
Mating film for dolicophallus: Example of apophallation (by the
first to withdraw). How costly is this for a hermaphrodite? It is
pretty rare: 5 out of 100 copulations end in apophallation.
Why does apophallation occur and when? They have observed it only nine
times and never by virgins. Is it done as retaliation if one partner does not give
any sperm? Do they run out, and perhaps later in life focus on egg laying?
Note in the table comparing copulations that dolicophallus and californicus are indistinguishable by mitochondrial DNA.
The rapid morphological changes are among the evidence for sex
selection, as well as the high cost of courtship/mating. There is
some evidence for sperm competition.
Soon we should have based on microsatellite data (nuclear DNA) …
It is unknown how many chromosomes they have, though chromosome variation is usually not seen till “quite high” taxonomic levels.
Their egg laying habits in the field are uncertain. Perhaps under
leaflitter. They do not dig holes in moist soil like garden
snails. In the lab at 19^C, they take seven weeks to hatch, sometimes two to three weeks longer. The record for a clutch is
seventy-five eggs (but this could have been multiple clutches since they don't check every day). Egg size varies. Dollicophallus
eggs are almost the size of jellybelly and weigh up to half a gram. For californicus they're usually under 0.3g.
Egg laying starts in fall and copulation is associated with foggy
nights (late summer, Santa Cruz). Eggs are laid October through
December when the rains come and usually finished by February. Hypothesis: Low pressure systems trigger egg laying. (This is based
on lab observations, and makes sense since dehydration is the highest mortality source. You want to lay eggs at the start of the rainy season
in a moist place.)
They have observed copulation for slugs as young as six months and egg laying as young as ten.
How long do they live? No one knows but perhaps two to three years. Some have lived thirty months in the lab (when sacrificed).