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lecture_notes:04-14-2010 [2010/04/17 05:59] jstjohn |
lecture_notes:04-14-2010 [2010/04/19 18:25] (current) galt |
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- The blunt ended fragments are A tailed specifically on the 3' ends of the DNA. | - The blunt ended fragments are A tailed specifically on the 3' ends of the DNA. | ||
- Double stranded library adapters have overhanging T which allows ligation to blunt ends in a specific orientation. | - Double stranded library adapters have overhanging T which allows ligation to blunt ends in a specific orientation. | ||
- | - This orientation may be used to insure single stranded samples | + | - This orientation may be used to ensure single stranded samples |
==== 454 Specific Library Construction ==== | ==== 454 Specific Library Construction ==== |