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lecture_notes:04-09-2010 [2010/04/11 22:30] learithe |
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For the most part Pog is done; however, there are still some uncertainty with 8 SNPs left. It is definitely past the MIAMI standard at this point. | For the most part Pog is done; however, there are still some uncertainty with 8 SNPs left. It is definitely past the MIAMI standard at this point. | ||
- | Note about sequencing platform quality scores: most platforms are trying to use the phred quality score((http://en.wikipedia.org/wiki/Phred_quality_score)), so the quality score is comparable between the platforms and runs | + | Note about sequencing platform quality scores: most platforms are trying to use the phred quality score((http://en.wikipedia.org/wiki/Phred_quality_score)), so the quality score is theoretically comparable between the platforms and runs (although calibration causes scores to vary between runs and instruments nonetheless) |
It can be informative, once reads are mapped, to look at the quality scores for reads with observed errors. | It can be informative, once reads are mapped, to look at the quality scores for reads with observed errors. |