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lecture_notes:04-06-2015

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lecture_notes:04-06-2015 [2015/04/09 22:44]
gepoliano
lecture_notes:04-06-2015 [2015/04/09 22:55] (current)
gepoliano
Line 171: Line 171:
  
 //ILLUMINA SEQUENCING//​ //ILLUMINA SEQUENCING//​
 +
 Different 3’ and 5’ end adapters – fragments are flanked by the adapters Different 3’ and 5’ end adapters – fragments are flanked by the adapters
 Hybridization in the flowcell (array) Hybridization in the flowcell (array)
Line 177: Line 178:
 A washing step takes out one of the two types A washing step takes out one of the two types
 Same cluster: same sequence, sequencing primer Same cluster: same sequence, sequencing primer
-Four nucleotides are labeled, all 4 in the same reaction, different 4 calors ​for each nucleotide +Four nucleotides are labeled, all 4 in the same reaction, different 4 colors ​for each nucleotide 
-Incorporation by polymerase – light release with colors +Incorporation by polymerase – light release with colors. 
-Same clusters – signal+Same clusters – signal.
 CDC camera catches the color. CDC camera catches the color.
 The process continues until ~100 bp The process continues until ~100 bp
 $1000 for a flowcell MinION – 400bp 1 lane  $1000 for a flowcell MinION – 400bp 1 lane 
  
- +
 //PACBIO// //PACBIO//
 +
 Imagine a plate with small wells. In this technology, the objective is to make a polymerase stick to the well (Eid et al.,  2009). This can be imagined as something like ELISA but with only one exactly DNA and polymerase per well. PACBIO is a Real Time sequencing technology, allowing the time it takes to incorporate the base, a measure to infer information about heterochromatin and quadruplex structures. This technology allows long reads. Howver indels are the main mismatch that happens in PacBio. Imagine a plate with small wells. In this technology, the objective is to make a polymerase stick to the well (Eid et al.,  2009). This can be imagined as something like ELISA but with only one exactly DNA and polymerase per well. PACBIO is a Real Time sequencing technology, allowing the time it takes to incorporate the base, a measure to infer information about heterochromatin and quadruplex structures. This technology allows long reads. Howver indels are the main mismatch that happens in PacBio.
  
lecture_notes/04-06-2015.1428644689.txt.gz · Last modified: 2015/04/09 22:44 by gepoliano