lecture_notes:04-06-2015
Differences
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lecture_notes:04-06-2015 [2015/04/10 05:44] gepoliano |
lecture_notes:04-06-2015 [2015/04/10 05:55] (current) gepoliano |
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| //ILLUMINA SEQUENCING// | //ILLUMINA SEQUENCING// | ||
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| Different 3’ and 5’ end adapters – fragments are flanked by the adapters | Different 3’ and 5’ end adapters – fragments are flanked by the adapters | ||
| Hybridization in the flowcell (array) | Hybridization in the flowcell (array) | ||
| Line 177: | Line 178: | ||
| A washing step takes out one of the two types | A washing step takes out one of the two types | ||
| Same cluster: same sequence, sequencing primer | Same cluster: same sequence, sequencing primer | ||
| - | Four nucleotides are labeled, all 4 in the same reaction, different 4 calors for each nucleotide | + | Four nucleotides are labeled, all 4 in the same reaction, different 4 colors for each nucleotide |
| - | Incorporation by polymerase – light release with colors | + | Incorporation by polymerase – light release with colors. |
| - | Same clusters – signal | + | Same clusters – signal. |
| CDC camera catches the color. | CDC camera catches the color. | ||
| The process continues until ~100 bp | The process continues until ~100 bp | ||
| $1000 for a flowcell MinION – 400bp 1 lane | $1000 for a flowcell MinION – 400bp 1 lane | ||
| - | + | ||
| //PACBIO// | //PACBIO// | ||
| + | |||
| Imagine a plate with small wells. In this technology, the objective is to make a polymerase stick to the well (Eid et al., 2009). This can be imagined as something like ELISA but with only one exactly DNA and polymerase per well. PACBIO is a Real Time sequencing technology, allowing the time it takes to incorporate the base, a measure to infer information about heterochromatin and quadruplex structures. This technology allows long reads. Howver indels are the main mismatch that happens in PacBio. | Imagine a plate with small wells. In this technology, the objective is to make a polymerase stick to the well (Eid et al., 2009). This can be imagined as something like ELISA but with only one exactly DNA and polymerase per well. PACBIO is a Real Time sequencing technology, allowing the time it takes to incorporate the base, a measure to infer information about heterochromatin and quadruplex structures. This technology allows long reads. Howver indels are the main mismatch that happens in PacBio. | ||
lecture_notes/04-06-2015.1428644689.txt.gz · Last modified: 2015/04/10 05:44 by gepoliano
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