Banana Slug Genomics

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====== Sequencing Technologies, Nader Pourmand ====== Nader detailed the procedures that were used to generate reads from the banana slug genome, as well as shortcomings that we would need to be aware of while processing the results: ===== Library Preparation ===== ==== Size selection ==== * On gel, DNA tangling causes size selection to be imprecise. * Instruments: SAGE, Caliper ===== Sequencing Technologies ===== * Sequencing by synthesis (454 and Illumina) vs sequencing by ligation (SOLiD) * Pyrosequencing (454) * Paired tag (Illumina and SOLiD)