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==== In-house ==== | ==== In-house ==== | ||
- | * Nader's lab uses a method that starts by fragmenting the DNA into long fragments (aiming for ~10k). They attach a unique barcode primer to each fragment and do circular ligation, much like SOLiD. This is followed by circular replication, primer binding, and random cleavage to obtain fragments of different lengths [(cite:circular_rep>http://en.wikipedia.org/wiki/Rolling_circle_replication)]. They sequence both ends of each fragment on the Illumina, obtaining a paired read with its 10k fragment barcode. | + | * Nader's lab uses a method that starts by fragmenting the DNA into long fragments (aiming for ~10k). They attach a unique barcode primer to each fragment and do circular ligation, much like SOLiD. This is followed by circular replication, primer binding, and random cleavage to obtain fragments of different lengths [(cite:circular_rep>http://en.wikipedia.org/wiki/Rolling_circle_replication)]. They sequence both ends of each fragment on the Illumina, obtaining a paired read with its 10k fragment's barcode. |