lecture_notes:04-01-2011
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lecture_notes:04-01-2011 [2011/04/03 18:55] eyliaw |
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| - | * Nader's lab uses a method that starts by fragmenting the DNA into long fragments (aiming for ~10k). They attach a unique primer to each fragment and do circular ligation, much like SOLiD. This is followed by circular replication, primer binding, and random cleavage to obtain fragments of different lengths [(cite:circular_rep>http://en.wikipedia.org/wiki/Rolling_circle_replication)]. They sequence both ends of each fragment. | + | * Nader's lab uses a method that starts by fragmenting the DNA into long fragments (aiming for ~10k). They attach a unique barcode primer to each fragment and do circular ligation, much like SOLiD. This is followed by circular replication, primer binding, and random cleavage to obtain fragments of different lengths [(cite:circular_rep>http://en.wikipedia.org/wiki/Rolling_circle_replication)]. They sequence both ends of each fragment on the Illumina, obtaining a paired read with its 10k fragment barcode. |
lecture_notes/04-01-2011.txt ยท Last modified: 2011/04/03 19:28 by eyliaw
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