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lecture_notes:03-31-2010 [2010/04/02 06:16] galt |
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**Mate-Pairing** - The chances of damage to DNA increases with time. Hence, there is a high possibility of not getting the accurate sequence data from the other end of DNA. To avoid this, the Mate-Pairing process involves the determination of sequence of DNA from the end towards the beginning and then from the beginning towards the end.\\ | **Mate-Pairing** - The chances of damage to DNA increases with time. Hence, there is a high possibility of not getting the accurate sequence data from the other end of DNA. To avoid this, the Mate-Pairing process involves the determination of sequence of DNA from the end towards the beginning and then from the beginning towards the end.\\ | ||
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- | ==== Alternative Notes ==== | ||
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- | === High Level Overview === | ||
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- | The high level sequencing workflow for all next gen tools is as follows: | ||
- | Fragment Sample -> Amplify Fragments -> Sequence Fragments | ||
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- | There are two main flavors of next generation sequencing technology: | ||
- | * Sequencing by Synthesis (SBS) | ||
- | * Hybridization | ||
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- | === Examination of Individual Technologies === | ||
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- | There are four technologies that were talked about today. | ||
- | * Pyrosequencing (454) | ||
- | * solid | ||
- | * Illumina/solexa | ||
- | * Ion Torrent | ||
== Pyrosequencing (SBS) == | == Pyrosequencing (SBS) == |