lecture_notes:03-31-2010
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| == Ion Torrent (SBS) == | == Ion Torrent (SBS) == | ||
| - | Works very similarly to pyrosequencing, except rather than requiring light from luciferase enzymes it takes advantage of the proton that is released when a nucleotide is added to a template. It measures this electric current. The presense of a current over a certain template when a specific nucleotide is added to solution tells you that that nucleotide was added to that template. The level of current at a template tells you the number of nucleotides that were added in that run. | + | Works very similarly to pyrosequencing, except rather than requiring light from luciferase enzymes it takes advantage of the proton that is released when a nucleotide is added to a template. It measures this electric current. The presence of a current over a certain template when a specific nucleotide is added to solution tells you that that nucleotide was added to that template. The level of current at a template tells you the number of nucleotides that were added in that run. |
| Can sequence about 60 bases every 100 minutes. | Can sequence about 60 bases every 100 minutes. | ||
lecture_notes/03-31-2010.txt ยท Last modified: 2010/04/13 14:24 by learithe
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