lecture_notes:03-31-2010
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| There is a problem with multiple A's because of the modified nucleotides which are needed for this reaction. FIXME | There is a problem with multiple A's because of the modified nucleotides which are needed for this reaction. FIXME | ||
| - | You must get the ammount of template on the beads just right. Must be all the same type. If too much template is present the read length is shortened (not sure why). If too few template is on the beads then the washing step will take away a significant amount of template and you won't have enough of a signal. | + | You must get the amount of template on the beads just right. Must be all the same type. If too much template is present the read length is shortened (not sure why). If too few template is on the beads then the washing step will take away a significant amount of template and you won't have enough of a signal. |
| The packing beads that are placed around the bead which holds the template strands in the individual wells on the plate are what hold the various enzymes (other than the polymerase) needed for the reaction. | The packing beads that are placed around the bead which holds the template strands in the individual wells on the plate are what hold the various enzymes (other than the polymerase) needed for the reaction. | ||
lecture_notes/03-31-2010.txt ยท Last modified: 2010/04/13 14:24 by learithe
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