lecture_notes:03-31-2010
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lecture_notes:03-31-2010 [2010/04/01 10:43] svasili |
lecture_notes:03-31-2010 [2010/04/02 06:07] galt |
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| **This post was copied from [[lecture_notes:Mar31|this original source location]]** | **This post was copied from [[lecture_notes:Mar31|this original source location]]** | ||
| - | Nader talked about the chemistry behind 4 Sequencing Platforms : SOLiD Bioanalyzer, 454, Illumina/Solexa, and charge based detection system Now sequencing.\\ | + | Nader talked about the chemistry behind 4 Sequencing Platforms : SOLiD Bioanalyzer, 454, Illumina/Solexa, and charge perturbation detection system Now sequencing.\\ |
| Sequencing workflow : Genomic DNA -> fragment -> amplification -> immobilization -> Sequencing.\\ | Sequencing workflow : Genomic DNA -> fragment -> amplification -> immobilization -> Sequencing.\\ | ||
| Time required for sequencing through these platforms is :\\ | Time required for sequencing through these platforms is :\\ | ||
| - | 1-2 hrs for CBD.\\ | + | 1-2 hrs for CPD.\\ |
| 9 hrs for 454.\\ | 9 hrs for 454.\\ | ||
| 3-7 days for Illumina/Solexa.\\ | 3-7 days for Illumina/Solexa.\\ | ||
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| The underlying technolgies for the platforms :\\ | The underlying technolgies for the platforms :\\ | ||
| - | * Sequencing by synthesis (SBS) : 454, Illumina/Solexa, Helicos, Pacbio, Charge based detection system Now Sequencing.\\ | + | * Sequencing by synthesis (SBS) : 454, Illumina/Solexa, Helicos, Pacbio, Charge Perturbation Detection system Now Sequencing.\\ |
| * Sequencing by ligation : SOLiD.\\ | * Sequencing by ligation : SOLiD.\\ | ||
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| Can sequence about 60 bases every 100 minutes. | Can sequence about 60 bases every 100 minutes. | ||
| - | Nader recommended reading the 2006 PNAS paper for background on this technology. | + | Nader recommended reading the [[http://www.pnas.org/content/103/17/6466.abstract|2006 PNAS paper]] for background on this technology. |
lecture_notes/03-31-2010.txt ยท Last modified: 2010/04/13 14:24 by learithe
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