lecture_notes:03-31-2010
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lecture_notes:03-31-2010 [2010/04/01 10:42] svasili |
lecture_notes:03-31-2010 [2010/04/01 18:17] galt |
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| ABI SOLiD sequencing technology follows sequencing by ligation. In first ligation cycle, possible 1024 oligonucleotide probes compete for incorporation, followed by dephosphorylation, visualization, and cleavage. Same procedure is followed for the next ligation cycles. Every 5th round of cycle, the process is reset. In the next cycle, primer length is reduced by one base (n-1) to get overlap, and again in the subsequent cycles the primer length is n-2, n-3 and so on to determine the DNA sequence due to overlap. For further reading follow [[http://sequencing.soe.ucsc.edu/node/20]].\\ | ABI SOLiD sequencing technology follows sequencing by ligation. In first ligation cycle, possible 1024 oligonucleotide probes compete for incorporation, followed by dephosphorylation, visualization, and cleavage. Same procedure is followed for the next ligation cycles. Every 5th round of cycle, the process is reset. In the next cycle, primer length is reduced by one base (n-1) to get overlap, and again in the subsequent cycles the primer length is n-2, n-3 and so on to determine the DNA sequence due to overlap. For further reading follow [[http://sequencing.soe.ucsc.edu/node/20]].\\ | ||
| - | **Mate-Pair** - The chances of damage to DNA increases with time. Hence, there is a high possibility of not getting the accurate sequence data from the other end of DNA. To avoid this, the Mate-Pair process involves the determination of sequence of DNA from the end towards the beginning and then from the beginning towards the end.\\ | + | **Mate-Pairing** - The chances of damage to DNA increases with time. Hence, there is a high possibility of not getting the accurate sequence data from the other end of DNA. To avoid this, the Mate-Pairing process involves the determination of sequence of DNA from the end towards the beginning and then from the beginning towards the end.\\ |
| ==== Alternative Notes ==== | ==== Alternative Notes ==== | ||
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| Can sequence about 60 bases every 100 minutes. | Can sequence about 60 bases every 100 minutes. | ||
| - | Nader recommended reading the 2006 PNAS paper for background on this technology. | + | Nader recommended reading the [[http://www.pnas.org/content/103/17/6466.abstract|2006 PNAS paper]] for background on this technology. |
lecture_notes/03-31-2010.txt ยท Last modified: 2010/04/13 14:24 by learithe
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