lecture_notes:03-30-2011
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lecture_notes:03-30-2011 [2011/03/30 23:31] eyliaw |
lecture_notes:03-30-2011 [2011/03/30 23:37] eyliaw [Illumina] |
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| * Pyrosequencing | * Pyrosequencing | ||
| * Q ~20 | * Q ~20 | ||
| - | * $5000/run/~1m reads, no downscaling (numbers approximate). | + | * $5000/run/1M reads, no downscaling (numbers approximate). |
| ==== SoLiD ==== | ==== SoLiD ==== | ||
| * 2x25bp or 1x50bp reads | * 2x25bp or 1x50bp reads | ||
| * Paired end reads: ligation with adapter, cleaves 25bp from adapter using restriction enzyme. | * Paired end reads: ligation with adapter, cleaves 25bp from adapter using restriction enzyme. | ||
| * Potential for double ligation: two unrelated sequences ligating. | * Potential for double ligation: two unrelated sequences ligating. | ||
| - | * $2000/run/100m reads (numbers approximate). | + | * $2000/run/100M reads (numbers approximate). |
| ==== Illumina ==== | ==== Illumina ==== | ||
| + | * 2x50, 2x100bps ? | ||
| + | * Paired end reads | ||
| + | * Potential errors: innies (ligated region not between sequenced regions) or chimeric (sequence passes ligated region) | ||
| + | * Cheaper than SoLiD, 10K Genomes project uses it. | ||
| ==== Ion Torrent ==== | ==== Ion Torrent ==== | ||
lecture_notes/03-30-2011.txt · Last modified: 2011/04/01 19:20 by svohr
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