lecture_notes:03-30-2011
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lecture_notes:03-30-2011 [2011/03/30 23:20] eyliaw [Sanger capillary] |
lecture_notes:03-30-2011 [2011/03/30 23:31] eyliaw |
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| ==== 454 ==== | ==== 454 ==== | ||
| * ~400bp reads[(cite:wiki454>http://en.wikipedia.org/wiki/454_Life_Sciences)]. | * ~400bp reads[(cite:wiki454>http://en.wikipedia.org/wiki/454_Life_Sciences)]. | ||
| - | * Q ~20 | ||
| * Pyrosequencing | * Pyrosequencing | ||
| - | * $5000/run/~1m reads, no downscaling. | + | * Q ~20 |
| + | * $5000/run/~1m reads, no downscaling (numbers approximate). | ||
| ==== SoLiD ==== | ==== SoLiD ==== | ||
| + | * 2x25bp or 1x50bp reads | ||
| + | * Paired end reads: ligation with adapter, cleaves 25bp from adapter using restriction enzyme. | ||
| + | * Potential for double ligation: two unrelated sequences ligating. | ||
| + | * $2000/run/100m reads (numbers approximate). | ||
| ==== Illumina ==== | ==== Illumina ==== | ||
lecture_notes/03-30-2011.txt · Last modified: 2011/04/01 19:20 by svohr
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