lecture_notes:03-30-2011
Differences
This shows you the differences between two versions of the page.
| Both sides previous revision Previous revision Next revision | Previous revision Next revision Both sides next revision | ||
|
lecture_notes:03-30-2011 [2011/03/30 23:10] eyliaw [Sanger capillary] |
lecture_notes:03-30-2011 [2011/03/30 23:20] eyliaw [Sanger capillary] |
||
|---|---|---|---|
| Line 6: | Line 6: | ||
| Inputs: Sequencing data from various machines. Some of the characteristics of these machines/techniques: | Inputs: Sequencing data from various machines. Some of the characteristics of these machines/techniques: | ||
| ==== Sanger capillary ==== | ==== Sanger capillary ==== | ||
| - | [(cite:sangerwiki>http://en.wikipedia.org/wiki/Microfluidic_Sanger_sequencing)] | + | * ~800bp reads[(cite:wikisanger>http://en.wikipedia.org/wiki/Microfluidic_Sanger_sequencing)]. |
| + | * Q (quality value) ~30 | ||
| + | * ~$1/read, expensive because primers must be attached to each read. | ||
| ==== 454 ==== | ==== 454 ==== | ||
| + | * ~400bp reads[(cite:wiki454>http://en.wikipedia.org/wiki/454_Life_Sciences)]. | ||
| + | * Q ~20 | ||
| + | * Pyrosequencing | ||
| + | * $5000/run/~1m reads, no downscaling. | ||
| ==== SoLiD ==== | ==== SoLiD ==== | ||
lecture_notes/03-30-2011.txt · Last modified: 2011/04/01 19:20 by svohr
Except where otherwise noted, content on this wiki is licensed under the following license: CC Attribution-Noncommercial-Share Alike 3.0 Unported
