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lecture_notes:03-30-2011 [2011/03/30 23:05] eyliaw |
lecture_notes:03-30-2011 [2011/03/30 23:20] eyliaw [Sanger capillary] |
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Inputs: Sequencing data from various machines. Some of the characteristics of these machines/techniques: | Inputs: Sequencing data from various machines. Some of the characteristics of these machines/techniques: | ||
- | ===== Sanger capillary ===== | + | ==== Sanger capillary ==== |
+ | * ~800bp reads[(cite:wikisanger>http://en.wikipedia.org/wiki/Microfluidic_Sanger_sequencing)]. | ||
+ | * Q (quality value) ~30 | ||
+ | * ~$1/read, expensive because primers must be attached to each read. | ||
+ | ==== 454 ==== | ||
+ | * ~400bp reads[(cite:wiki454>http://en.wikipedia.org/wiki/454_Life_Sciences)]. | ||
+ | * Q ~20 | ||
+ | * Pyrosequencing | ||
+ | * $5000/run/~1m reads, no downscaling. | ||
+ | ==== SoLiD ==== | ||
- | 454 | + | ==== Illumina ==== |
- | SoLiD | + | |
- | Illumina | + | ==== Ion Torrent ==== |
- | Ion Torrent | + | |
- | Pac Bio | + | ==== Pac Bio ==== |
+ | |||
+ | ===== References ===== | ||
+ | <refnotes>notes-separator: none</refnotes> | ||
+ | ~~REFNOTES cite~~ |