data_overview:2015:rna-seq
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data_overview:2015:rna-seq [2015/08/31 20:39] ceisenhart |
data_overview:2015:rna-seq [2015/08/31 20:48] ceisenhart |
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| ====== Banana Slug RNA-Seq ====== | ====== Banana Slug RNA-Seq ====== | ||
| - | ===== Sequencing data ===== | + | ===== Raw data ===== |
| - | | Library | Run | Location | Notes | | + | The raw data can be accessed online [[http://genome-test.cse.ucsc.edu/~charles/BME235_backup/Data/2015/rnaSeq/ | here]]. |
| - | | - | - | http://genome-test.cse.ucsc.edu/~charles/BME235_backup/Data/2015/rnaSeq/ | | | + | |
| - | + | ||
| - | ===== Files ===== | + | |
| - | | File | Size | Reads| | + | |
| - | |BS-AL-SmartSeq_S9_L005_R1_001.fastq.gz | 7.4G | | | + | |
| - | |BS-AL-SmartSeq_S9_L005_R2_001.fastq.gz | 7.4G | | | + | |
| - | |BS-AL-Tn5Prime_S7_L005_R1_001.fastq.gz | 1.1G | | | + | |
| - | |BS-AL-Tn5Prime_S7_L005_R2_001.fastq.gz | 1.1G | | | + | |
| - | |BS-PA-SmartSeq_S10_L005_R1_001.fastq.gz | 7.8G | | | + | |
| - | |BS-PA-SmartSeq_S10_L005_R2_001.fastq.gz | 7.8G | | | + | |
| - | |BS-PA-Tn5Prime_S6_L005_R1_001.fastq.gz | 2.2G | | | + | |
| - | |BS-PA-Tn5Prime_S6_L005_R2_001.fastq.gz | 2.2G | | | + | |
| - | |BS-P-SmartSeq_S11_L005_R1_001.fastq.gz | 6.5G | | | + | |
| - | |BS-P-SmartSeq_S11_L005_R2_001.fastq.gz | 6.5G | | | + | |
| - | |BS-P-Tn5Prime_S8_L005_R1_001.fastq.gz | 3.0G | | | + | |
| - | |BS-P-Tn5Prime_S8_L005_R2_001.fastq.gz | 3.0G | | | + | |
| Total = 56G Raw unprocessed data | Total = 56G Raw unprocessed data | ||
| ===== FastQC ===== | ===== FastQC ===== | ||
| - | The fastQC reports for the above files can be found online here [[http://hgwdev-ceisenhart.cse.ucsc.edu/~charles/BME235_backup/Data/2015/rnaSeq/fastQC/ | raw fastqc reports]]. | + | The fastQC reports for the raw files can be found online here [[http://hgwdev-ceisenhart.cse.ucsc.edu/~charles/BME235_backup/Data/2015/rnaSeq/fastQC/ | raw fastqc reports]]. |
| ===== Processed data ===== | ===== Processed data ===== | ||
| - | [[data_overview:2015:rna-seq:fastUniq | fastUniq ]] | + | FastQC reports for the processed files can be found through the ftp links below. |
| - | [[data_overview:2015:rna-seq:fastUniq | fastUniq + fastqQuality ]] | + | |
| + | [[http://hgwdev-ceisenhart.cse.ucsc.edu/~charles/BME235_backup/Data/2015/rnaSeq/fastUniq/ | fastUniq]] fastUniq removes PCR duplicates. | ||
| + | |||
| + | |||
| + | [[http://hgwdev-ceisenhart.cse.ucsc.edu/~charles/BME235_backup/Data/2015/rnaSeq/fastUniq/fastqQuality | fastUniq + fastqQuality ]] fastqQuality removes low quality reads. | ||
| - | Libraries are completed. Preliminary data is undergoing analysis. | ||
| ===== RNA Extraction ===== | ===== RNA Extraction ===== | ||
| RNA was extracted from three tissues, the albumen, proximal albumen and penis. The protocol used for RNA extraction is as follows: | RNA was extracted from three tissues, the albumen, proximal albumen and penis. The protocol used for RNA extraction is as follows: | ||
data_overview/2015/rna-seq.txt · Last modified: 2015/09/02 16:12 by ceisenhart
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