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data_overview:2015:rna-seq [2015/08/03 18:12]
ceisenhart
data_overview:2015:rna-seq [2015/09/02 16:11]
ceisenhart Adding backlinks
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 ====== Banana Slug RNA-Seq ====== ====== Banana Slug RNA-Seq ======
 +To see how we are using this data, please view [[https://​banana-slug.soe.ucsc.edu/​post-assembly_analysis:​2015:​rna_scaffolding | this page]. ​
  
-===== Files ===== +===== Raw data ===== 
-File | Size | Reads|  +The raw data can be accessed online [[http://​genome-test.cse.ucsc.edu/​~charles/​BME235_backup/​Data/​2015/​rnaSeq/ ​here]]. ​  
-SW019_S1_L001_R1_001.fastq | 17G | 27,​268,​723 ​|+ 
 +Total = 56G Raw unprocessed data 
 + 
 +===== FastQC ===== 
 +The fastQC reports for the raw files can be found online here [[http://​hgwdev-ceisenhart.cse.ucsc.edu/​~charles/​BME235_backup/​Data/​2015/​rnaSeq/​fastQC/ ​raw fastqc reports]]. ​ 
 + 
 +===== Processed data ===== 
 + 
 +[[http://​hgwdev-ceisenhart.cse.ucsc.edu/​~charles/​BME235_backup/​Data/​2015/​rnaSeq/​fastUniq/ ​fastUniq]] fastUniq removes PCR duplicates 
 + 
 + 
 +[[http://​hgwdev-ceisenhart.cse.ucsc.edu/​~charles/​BME235_backup/​Data/​2015/​rnaSeq/​fastUniq/​fastqQuality ​fastUniq + fastqQuality ]] fastqQuality removes low quality reads.  
 + 
 +FastQC reports for the processed files can be found in the '​fastQC'​ subdirectories.  ​
  
  
-Libraries are completed. Preliminary data is undergoing analysis. 
 ===== RNA Extraction ===== ===== RNA Extraction =====
 RNA was extracted from three tissues, the albumen, proximal albumen and penis. The protocol used for RNA extraction is as follows: RNA was extracted from three tissues, the albumen, proximal albumen and penis. The protocol used for RNA extraction is as follows:
data_overview/2015/rna-seq.txt · Last modified: 2015/09/02 16:12 by ceisenhart