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data_overview:2015:rna-seq [2015/07/16 18:50]
ceisenhart created
data_overview:2015:rna-seq [2015/09/02 16:11]
ceisenhart Adding backlinks
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 ====== Banana Slug RNA-Seq ====== ====== Banana Slug RNA-Seq ======
-Libraries ​are completedPreliminary ​data is undergoing analysis.+To see how we are using this data, please view [[https://​banana-slug.soe.ucsc.edu/​post-assembly_analysis:​2015:​rna_scaffolding | this page].  
 + 
 +===== Raw data ===== 
 +The raw data can be accessed online [[http://​genome-test.cse.ucsc.edu/​~charles/​BME235_backup/​Data/​2015/​rnaSeq/​ | here]]. ​  
 + 
 +Total = 56G Raw unprocessed data 
 + 
 +===== FastQC ===== 
 +The fastQC reports for the raw files can be found online here [[http://​hgwdev-ceisenhart.cse.ucsc.edu/​~charles/​BME235_backup/​Data/​2015/​rnaSeq/​fastQC/​ | raw fastqc reports]].  
 + 
 +===== Processed data ===== 
 + 
 +[[http://​hgwdev-ceisenhart.cse.ucsc.edu/​~charles/​BME235_backup/​Data/​2015/​rnaSeq/​fastUniq/​ | fastUniq]] fastUniq removes PCR duplicates.  
 + 
 + 
 +[[http://​hgwdev-ceisenhart.cse.ucsc.edu/​~charles/​BME235_backup/​Data/​2015/​rnaSeq/​fastUniq/​fastqQuality | fastUniq + fastqQuality ]] fastqQuality removes low quality reads.  
 + 
 +FastQC reports for the processed files can be found in the '​fastQC'​ subdirectories  
 + 
 ===== RNA Extraction ===== ===== RNA Extraction =====
 RNA was extracted from three tissues, the albumen, proximal albumen and penis. The protocol used for RNA extraction is as follows: RNA was extracted from three tissues, the albumen, proximal albumen and penis. The protocol used for RNA extraction is as follows:
data_overview/2015/rna-seq.txt · Last modified: 2015/09/02 16:12 by ceisenhart