data_overview:2015:rna-seq
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data_overview:2015:rna-seq [2015/07/16 18:50] ceisenhart created |
data_overview:2015:rna-seq [2015/08/31 20:48] ceisenhart |
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| ====== Banana Slug RNA-Seq ====== | ====== Banana Slug RNA-Seq ====== | ||
| - | Libraries are completed. Preliminary data is undergoing analysis. | + | |
| + | ===== Raw data ===== | ||
| + | The raw data can be accessed online [[http://genome-test.cse.ucsc.edu/~charles/BME235_backup/Data/2015/rnaSeq/ | here]]. | ||
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| + | Total = 56G Raw unprocessed data | ||
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| + | ===== FastQC ===== | ||
| + | The fastQC reports for the raw files can be found online here [[http://hgwdev-ceisenhart.cse.ucsc.edu/~charles/BME235_backup/Data/2015/rnaSeq/fastQC/ | raw fastqc reports]]. | ||
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| + | ===== Processed data ===== | ||
| + | FastQC reports for the processed files can be found through the ftp links below. | ||
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| + | [[http://hgwdev-ceisenhart.cse.ucsc.edu/~charles/BME235_backup/Data/2015/rnaSeq/fastUniq/ | fastUniq]] fastUniq removes PCR duplicates. | ||
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| + | [[http://hgwdev-ceisenhart.cse.ucsc.edu/~charles/BME235_backup/Data/2015/rnaSeq/fastUniq/fastqQuality | fastUniq + fastqQuality ]] fastqQuality removes low quality reads. | ||
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| ===== RNA Extraction ===== | ===== RNA Extraction ===== | ||
| RNA was extracted from three tissues, the albumen, proximal albumen and penis. The protocol used for RNA extraction is as follows: | RNA was extracted from three tissues, the albumen, proximal albumen and penis. The protocol used for RNA extraction is as follows: | ||
data_overview/2015/rna-seq.txt · Last modified: 2015/09/02 16:12 by ceisenhart
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