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--- assemblies:2015:mitochondrion_assembly [2015/07/27 20:09]
karplus [Assembly] Added info about large mollusk mitochondrial genome.
+++ assemblies:2015:mitochondrion_assembly [2015/09/16 01:30] (current)
ndudek [PCR results]
@@ Line 209: / Line 209: @@
 {{:assemblies:2015:longest_vs_a_caerulea.png?200|}}
+August 2015
+PCR products were sent for Sanger sequencing. Something went wrong - the result was largely "N"s. I checked whether I could improve calls by looking at the chromatogram, but did not have significant success. Below is an example of what the chromatogram looked like. I will be re-sending the PCR products for sequencing.
+{{:assemblies:2015:chromatogram_mito_august2015.png?200|}}
+September 2015
+PCR products were sent for sequencing a second time, this time after a size selection on the strongest band. Results were essentially the same.
 The mitochondrion assembly is being worked on by Natasha Dudek (natasha@dudek.org) from Team 5: Discovar //de novo//.

Banana Slug Genomics