archive:specific_task
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archive:specific_task [2015/05/08 22:09] nsaremi |
archive:specific_task [2015/06/01 19:22] jdhouser |
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| |Task| Assignee | Status | Notes| | |Task| Assignee | Status | Notes| | ||
| - | | Map lucigen mates against scaffolds to estimate insert size | | | | | + | |Install Maker| Houser | In progress | Working on getting dependencies installed | |
| - | | Plot contig size vs total genome size for assemblies | | | | | + | |Assemble repeat genome with RepArk|Team 1|In progress|Charles has started a test run using the original Hiseq and Miseq reads| |
| - | | Ask UCSF about the discrepancy in the numbers of reads | | | | | + | |Run GapCloser on SOAP denovo assembly|Charles|In progress| Use SOAP denovo's external gap closer. Chose a reasonable name for new assembly. | |
| + | |Find virus contigs and use PRICE to expand to full viruses | Cole,jolespin | | jolespin virus assembly progress can be accessed at [[jolespin_virus]]| | ||
| + | | Mate-pair adapter and linker removal| | | | | ||
| + | | Measuring insert size for each mate-pair library | unassigned | | | | ||
| + | | Making Seqprep, adapter removed, end-trimmed library files | unassigned | | | | ||
| + | | Blast long contigs and analyze results | unassigned| | Check the browser for the 30 longest contigs from each group with contigs| | ||
| + | | Find genes in the mitochondrial genome | unassigned | | The genome is on the browser, find it here https://banana-slug.soe.ucsc.edu/banana_slug_genome_browser | | ||
| + | | Use PRICE to get mitochondrial genome as single contig | Natasha Dudek? | | | | ||
| + | | Plot contig size vs total genome size for assemblies | unassigned | | | | ||
| + | | Ask UCSF about the discrepancy in the numbers of reads | unassigned | | I don't see any discrepancy in the number of reads, perhaps this can be removed? | | ||
| + | | Fastqc/kmergenie on SeqPrepped data | unassigned | waiting on previous steps | | | ||
| + | | End trim SeqPrepped data | Hussain | | | | ||
| + | | Error correct SeqPrepped data | McGovern | waiting on previous step | | | ||
| + | | Error Correction new data| McGovern (Musket) | In progress | | | ||
| + | | Install bowtie | Chaves | In progress |/campusdata/gchaves/Bowtie/bowtie2-2.2.5 (Not yet running successfully)| | ||
| + | | UCSC Genome browser hub | Eisenhart | Complete | https://banana-slug.soe.ucsc.edu/banana_slug_genome_browser | | ||
| + | | Plot contig size histogram for current assembly (SOAPdenovo2 results) | Markello | Complete | | | ||
| + | | Plot scaffold size histogram for current assembly (SOAPdenovo2 results) | Markello | Complete | | | ||
| | Find cost of creating a tagmentase library| Dudek | | ~$50 | | | Find cost of creating a tagmentase library| Dudek | | ~$50 | | ||
| - | | Merge/trim new data (SeqPrep) | Hussain | in progress | | | + | | Merge/trim new data (SeqPrep) | Hussain | Completed | See wiki page for each data set for details | |
| - | | Analyze new data (preqc) | | | | | + | | Analyze new data (preqc) | Richardson | Completed | See wiki page for each data set for details | |
| - | | Analyze new data (fastqc) | Saremi |Completed | | | + | | Preqc on new mate pair SW041 SW042| Saremi | Completed | | |
| - | | Analyze SeqPrep results | | | | | + | | Update the wiki to include mate pair meta data | Eisenhart | completed | |
| - | | Error Correction new data| | | | | + | | Install CEGMA dependencies | Markello | Completed | Issue discovered: glib required for complete installation of latest version of genewise (CEGMA dependency) on head node, will use current installation of genewise. | |
| - | | Adapter trim new data| | | this will be in addition to the seqprep adapter removal | | + | | Create a file containing contigs longer than 5kb | Eisenhart | completed | file is here /campusdata/BME235/S15_assemblies/DiscovarDeNovo/UCSF50%run/bigContigs.fa | |
| - | + | | Plot scaffold size histogram for current assembly (Discovar de novo results) | Dudek | Completed | 10% and the new UCSF 50% are up on the discovar team page | | |
| - | + | | Map lucigen mates against scaffolds | Dudek | completed | See sam file at /campusdata/ndudek/mapping_lucigen/5kb_plus_discovar/IJS8_mates_vs_discovar_bigContigs_5000.sam | | |
| - | + | | Analyze new data (fastqc) | Saremi |Completed | /campusdata/BME235/S15_assemblies/SOAPdenovo2/Fastqc/UCSF_BS-MK_fastqc /campusdata/BME235/S15_assemblies/SOAPdenovo2/Fastqc/UCSF_BS-tag_fastqc | | |
| + | | Adapter trim UCSF SW018 and SW019 libraries (skewer), remove duplicates (fastUniq)| Chaves & Dudek | Completed |/campusdata/BME235/S15_assemblies/DiscovarDeNovo/UCSF_reads (Read 1 Sequencing Primer: 5' ACACTCTTTCCCTACACGACGCTCTTCCGATCT 3'; Read 2 Sequencing Primer: 5' GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT 3)| | ||
| + | |SSpace on discovar de novo assembly fasta | Eisenhart | Complete | Results are posted here , https://banana-slug.soe.ucsc.edu/team_5_page:sspacesummaryfile | | ||
| + | | Map reads against 2012 mitochondrion assembly | Dudek | Completed for UCSF SW018 and SW019 reads | I did the mapping both for adapter-trimmed and adapter-trimmed & duplicate-free reads. The sam files are available at: /campusdata/BME235/mitochondrion | | ||
archive/specific_task.txt · Last modified: 2015/08/04 03:32 by 68.180.228.52
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