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=====Banana Slug RNA===== =====RNA isolates===== In Feburary 2015 the [[http://omegabiotek.com/store/product/e-z-n-a-mollusc-rna-kit/|Omega Bio Tek E.Z.N.A.® Mollusc RNA kit]] was used to prepare 28 isolations of RNA from 16 tissue samples. In April 2015 the [[http://www.lifetechnologies.com/order/catalog/product/AM1561|mirVana™ miRNA Isolation Kit, without phenol]] was used to create a size selected sample of one of the RNA isolates (dg2). Later that month 9 RNA samples (V2, K, SMT, DA2, PA1, DG3, DG1, DG2-S, DG2-L) were run on a 1.5% CE Agarose gel. Unfortunately there was a miss-communication between Jared and his supervisor it is unknown which sample made it to what well. {{ :rnagel.png?nolink |}} =====RNA samples===== Abbreviations for tissues cuts: | Tissue cut | Abbreviation ID | number of cuts from tissue | | Albumen gland (proximal) | pa | 2 | | Albumen gland (distal) | da | 2 | | Penis | p | 3 | | Digestive Gland | dg | 3 | | Vagina | v | 2 | | Penis Muscle | pm | 2 | | Foot | f | 2 | | Salivary Gland | sg | 2 | | Ovotestis | ot | 2 | | Body Wall | bw | 2 | | Shell | sh | 1 | | Vas Deferons | vd | 1 | | Ovoduct | od | 1 | | Connective tissue | ct | 1 | | spermathecca | smt | 1 | | Kidney | k | 1 | The following table of data was generated with a [[http://www.nanodrop.com/Productnd8000overview.aspx?gclid=CJaNntn0sMUCFU6VfgodXI4AlQ|nanodrop]] | Sample ID | Conc. (ng/ul)| 260/280 | 260/230 | Vol. (ul)| Total (ug)| notes | | pa1 | 180.9 | 2 | 1.87 | 64.4 | 11.65 | | | pa2 | 279.3 | 1.99 | 2.32 | 70 | 19.55 | | | da1 | 150.2 | 2.04 | 1.36 | 70 | 10.51 | | | da2 | 113.4 | 2.09 | 0.23 | 61 | 6.93 | contaminated? | | v1 | 112 | 2.02 | 0.97 | 70 | 7.84 | tougher, lighter, bulbous | | v2 | 78.42 | 2.03 | 0.83 | 56 | 4.39 | flimsy, darker, sleeve-like | | pm1 | 3.207 | 1.91 | 0.41 | 70 | 0.22 | | | pm2 | 25.79 | 1.95 | 0.31 | 70 | 2.86 | | | f1 | 40.84 | 1.88 | 0.89 | 70 | 2.86 | | | f2 | 47.03 | 1.84 | 1.77 | 69 | 3.25 | | | sg1 | | sg2 | | ot1 | | ot2 | | bw1 | | bw2 | | sh | | vd | | od | | ct | | smt | | k | | p1 | | p2 | | p3 | | dg1 | | dg2 | | dg3 | | dg2-S | | dg2-L |
Discussion
I would like to flag this page for removal (or burial somewhere deep in the wiki), I don't see any use for the page when we don't have labeled lanes.
just bury this page as old_RNA or something as you add new data here.
How are we going to use these data when we don't know which lanes are which? Maybe I am misunderstanding this… “Unfortunately there was a miss-communication between Jared and his supervisor it is mostly unknown which sample made it to what well.”