archive:rna
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archive:rna [2015/05/08 02:55] JaredC |
archive:rna [2015/07/28 05:58] (current) ceisenhart ↷ Page moved from rna to archive:rna |
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| In Feburary 2015 the [[http://omegabiotek.com/store/product/e-z-n-a-mollusc-rna-kit/|Omega Bio Tek E.Z.N.A.® Mollusc RNA kit]] was used to prepare 28 isolations of RNA from 16 tissue samples. Note that this kit is not recommended for RNA <200nt and there was no DNase treatment. | In Feburary 2015 the [[http://omegabiotek.com/store/product/e-z-n-a-mollusc-rna-kit/|Omega Bio Tek E.Z.N.A.® Mollusc RNA kit]] was used to prepare 28 isolations of RNA from 16 tissue samples. Note that this kit is not recommended for RNA <200nt and there was no DNase treatment. | ||
| - | In April 2015 the [[http://www.lifetechnologies.com/order/catalog/product/AM1561|mirVana™ miRNA Isolation Kit, without phenol]] was used to create a size selected sample of one of the RNA isolates (dg2). Later that month 9 RNA samples (V2, K, SMT, DA2, PA1, DG3, DG1, DG2-S, DG2-L) were run on a 1.5% CE Agarose gel compared to a [[https://www.neb.com/products/n3231-100-bp-dna-ladder|NEB 100nt DNA ladder]]. Unfortunately there was a miss-communication between Jared and his supervisor it is mostly unknown which sample made it to what well. Note that there is still a presence of small RNA (<200 nt) but no distinct rRNA, tRNA, or miRNA bands. | + | In April 2015 the [[http://www.lifetechnologies.com/order/catalog/product/AM1561|mirVana™ miRNA Isolation Kit, without phenol]] was used to create a size selected sample of one of the RNA isolates (dg2). Later that month 9 RNA samples (V2, K, SMT, DA2, PA1, DG3, DG1, DG2-S, DG2-L) were run on a 1.5% CE Agarose gel compared to a [[https://www.neb.com/products/n3231-100-bp-dna-ladder|NEB 100nt DNA ladder]]. Unfortunately there was a miscommunication between Jared and his supervisor it is mostly unknown which sample made it to what well. Note that there is still a presence of small RNA (<200 nt) but no distinct rRNA, tRNA, or miRNA bands. |
| If we decide to make small RNA libraries a different protocol will need to be run on one of the left-over tissue cuts. | If we decide to make small RNA libraries a different protocol will need to be run on one of the left-over tissue cuts. | ||
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| | ct | 40.43 | 1.92 | 1.82 | 70 | 2.83 | | | | ct | 40.43 | 1.92 | 1.82 | 70 | 2.83 | | | ||
| | smt | 93.26 | 1.95 | 1.86 | 59 | 5.50 | Steven spilled some | | | smt | 93.26 | 1.95 | 1.86 | 59 | 5.50 | Steven spilled some | | ||
| - | | k | 160 | 1.98 | 2.11 | 63.5 | 10.16 | | | + | | k | 160.0 | 1.98 | 2.11 | 63.5 | 10.16 | | |
| | p1 | 53.08 | 1.89 | 1.59 | 70 | 3.72 | only epiphallus | | | p1 | 53.08 | 1.89 | 1.59 | 70 | 3.72 | only epiphallus | | ||
| | p2 | 16.91 | 1.75 | 1.06 | 70 | 1.18 | | | | p2 | 16.91 | 1.75 | 1.06 | 70 | 1.18 | | | ||
archive/rna.1431053737.txt.gz · Last modified: 2015/05/08 02:55 by JaredC
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