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===== Solid Sequencing Run 1 ===== ==== Meta ==== Prepared by: Eveline Hesson Date: Overview: ==== DNA Extraction ==== ==== Library Preparation ==== This library was prepared by following ABI's standard 2x25 mate-pair library preparation protocol. {{:lab_protocols:solid3_libprep_guide.pdf|}} Detailed notes of the protocol can be found in the following pdf: {{:lab_protocols:solid-mate-pair-notes.pdf|}} === Shearing DNA === Extracted genomic DNA was sheared using a [[https://products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=catNavigate2&catID=604432|Hydroshear]] to an approximate size of 4-5kb. The shearing was done using Standard_Assembly, sc 15, 5 cycles for 150 µl total volume. Total recovered DNA after hydroshear was 217 µl or 26 µg. === Repair the DNA Ends === The DNA ends of sheared gDNA were blunt ended and phosphorylated using Epicentre's End-It DNA repair Kit. 217 µl of sheared gDNA was reacted in a total volume of 400 µl according to manufacturer's standards. The reaction was incubated for 30 minutes at room temperature. === Purify the End-Repaired DNA using the QIAquick Gel Extraction Kit === ==== Emulsion PCR ==== ==== Sequencing Data ====
Discussion
Who did each of the steps of the protocol? dates?