Banana Slug Genomics

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===== Solid Sequencing Run 1 =====
==== Meta ====

Prepared by: Eveline Hesson

Date:

Overview:

==== DNA Extraction ====
==== Library Preparation ====

This library was prepared by following ABI's standard 2x25 mate-pair library preparation protocol. {{:lab_protocols:solid3_libprep_guide.pdf|}}

Detailed notes of the protocol can be found in the following pdf: {{:lab_protocols:solid-mate-pair-notes.pdf|}}

=== Shearing DNA ===
Extracted genomic DNA was sheared using a [[https://products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=catNavigate2&catID=604432|Hydroshear]] to an approximate size of 4-5kb. The shearing was done using Standard_Assembly, sc 15, 5 cycles for 150 µl total volume.

Total recovered DNA after hydroshear was 217 µl or 26 µg.

=== Repair the DNA Ends ===

The DNA ends of sheared gDNA were blunt ended and phosphorylated using Epicentre's End-It DNA repair Kit. 217 µl of sheared gDNA was reacted in a total volume of 400 µl according to manufacturer's standards. The reaction was incubated for 30 minutes at room temperature.

=== Purify the End-Repaired DNA using the QIAquick Gel Extraction Kit ===



==== Emulsion PCR ====

==== Sequencing Data ====

Banana Slug Genomics

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