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====== SeqPrep ====== SeqPrep is a program to merge paired end Illumina reads that are overlapping into a single longer read. It may also just be used for its adapter trimming feature without doing any paired end overlap. When an adapter sequence is present, that means that the two reads must overlap (in most cases) so they are forcefully merged. When reads do not have adapter sequence they must be treated with care when doing the merging, so a much more sensitive approach is taken. The default parameters were chosen with sensitivity in mind, so that they could be ran on libraries where very few reads are expected to overlap. It is always safest though to save the overlapping procedure for libraries where you have some prior knowledge that a significant portion of the reads will have some overlap. ===== Overview ===== SeqPrep works by first searching for adapter sequences at the ends of reads. If these adapter sequences are found it trims them off. If read merging is desired it will then force a merge between the two reads because if an adapter is present then the insert size must be less than the total read length. ==== Required Input ==== fastq reads in phred+33 or phred+64 format. If phred+64 format is supplied the argument -6 must be supplied and the output is converted into phred+33 format. ==== Output ==== ===== Installation ===== To install SeqPrep download it and run `make`. A binary is created which can then be copied to the location of your choice. By default `make install` places the binary in your $HOME/bin folder. ===== Example Run =====
