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--- archive:bioinformatic_tools:mira [2010/04/12 21:28]
mpcusack
+++ archive:bioinformatic_tools:mira [2015/09/04 09:06] (current)
68.180.228.52 ↷ Links adapted because of a move operation
@@ Line 1: / Line 1: @@
-[[http://sourceforge.net/apps/mediawiki/mira-assembler/index.php?title=Main_Page||External Page]] - Contains Description and download links.
+[[http://sourceforge.net/apps/mediawiki/mira-assembler/index.php?title=Main_Page||Sourceforge Page]] - Contains description, documentation and download links.
+MIRA works with Sanger, 454 and Illumina data. It is 12 years in the making and still being developed.
+[[http://www.chevreux.org/thesis/index.html|Chevreux, B. (2005). //MIRA: An Automated Genome and EST Assembler.//
+ Ph.D. thesis, The Medical Faculty of Heidelberg of the Ruprecht-Karls-
+ University.]]
-MIRA works with Sanger, 454 and Illumina data. It is 12 years in the making and still being actively developed.
 ====Installation====
-To install I needed to first download and install GNU Flex 2.5.35 and Expat libraries.
+To install we first needed to download and install [[http://flex.sourceforge.net/|GNU Flex]] 2.5.35 and [[http://sourceforge.net/projects/expat/|Expat]] 2.0.1 libraries.
 Both were downloaded from sourceforge.net and installed with
   ./configure --prefix=/campusdata/BME235/
@@ Line 10: / Line 15: @@
   make install
-Once those were both installed I could run configure with
+Once the prerequisites were installed, installation was successful with with
   ./configure --prefix=/campusdata/BME235 --with-boost=/campusdata/BME235 --with-boost-libdir=/campusdata/BME235/lib --with-expat=/campusdata/BME235
+  make
+  make install
+This added the following binaries to the [[archive:computer_resources:bin|bin]] directory
+<code>-rwxr-xr-x+  1 mcusack  bme235  3.0M Apr 11 21:18 convert_project
+-rwxr-xr-x+  1 mcusack  bme235   11K Apr 11 21:18 fasta2frag.tcl
+-rwxr-xr-x+  1 mcusack  bme235  3.2K Apr 11 21:18 fastaselect.tcl
+-rwxr-xr-x+  1 mcusack  bme235   86K Apr 11 21:18 fastatool
+-rwxr-xr-x+  1 mcusack  bme235  1.6K Apr 11 21:18 fixACE4consed.tcl
+-rwxr-xr-x+  1 mcusack  bme235  3.1M Apr 11 21:18 mira
+lrwxrwxrwx   1 mcusack  bme235     4 Apr 11 21:18 miraclip -> mira
+lrwxrwxrwx   1 mcusack  bme235     4 Apr 11 21:18 miramem -> mira
+lrwxrwxrwx   1 mcusack  bme235     4 Apr 11 21:18 miraSearchESTSNPs -> mira
+-rwxr-xr-x+  1 mcusack  bme235  568K Apr 11 21:18 scftool
+</code>
+====Internals===
+Slides from a presentation dated 5/17 by Michael Cusack summarizing how MIRA works {{:bioinformatic_tools:mira_internals.pdf|}}.
+====Assemblies===
+A list of assemblies using MIRA can be found [[archive:computer_resources:assemblies:mira|here]].
+Currently just two assembies have been performed with MIRA using //Pyrobaculum oguniense// data.
+====Conclusions====
+MIRA appears to be a very good assembler for denovo data. It's strengths are that it can take in many types of data tries to use all data to the fullest while keeping quality of the results high. Based on tests there appears to be occasional problems with chimeric contigs in the results, but this was fixed by specifying more stringent settings.
+Disadvantages are mainly its speed and memory requirements. Assembling Pog took 13 hours, and mira estimates it would need 1.1 TB of memory to assemble the banana slug (using data available May 2010). Documentation is also spotty and often outdated. The thesis referenced on this page is the most complete reference of how MIRA works, but it is several years (and versions) old. Some later documentation mentions that parts of the processing have changed in recent versions, but do not specify what or how.

Banana Slug Genomics

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